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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3-4
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pubmed:dateCreated |
2007-6-26
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pubmed:abstractText |
Prions are composed solely of the disease-causing prion protein (PrPSc) that is formed from the cellular isoform PrPC by a posttranslational process. Here we report that short phosphorothioate DNA (PS-DNA) oligonucleotides diminished the levels of both PrPC and PrPSc in prion-infected neuroblastoma (ScN2a) cells. The effect of PS-DNA on PrP levels was independent of the nucleotide sequence. The effective concentration (EC50) of PS-DNA required to achieve half-maximal diminution of PrPSc was approximately 70 nM, whereas the EC50 of PS-DNA for PrPC was more than 50-fold greater. This finding indicated that diminished levels of PrPSc after exposure to PS-DNA are unlikely to be due to decreased PrPC levels. Bioassays in transgenic mice demonstrated a substantial diminution in the prion infectivity after ScN2a cells were exposed to PS-DNAs. Whether PS-DNA will be useful in the treatment of prion disease in people or livestock remains to be established.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-10525406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-10588739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-10756052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-10775631,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-12621436,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-2776777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-3282080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-3693391,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-8790598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-9212101,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-9356250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592554-9765500
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1076-1551
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
190-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17592554-Animals,
pubmed-meshheading:17592554-Mice,
pubmed-meshheading:17592554-Phosphates,
pubmed-meshheading:17592554-Fluorescent Dyes,
pubmed-meshheading:17592554-Neuroblastoma,
pubmed-meshheading:17592554-Fluoresceins,
pubmed-meshheading:17592554-Tumor Cells, Cultured,
pubmed-meshheading:17592554-Time Factors,
pubmed-meshheading:17592554-Cell Survival,
pubmed-meshheading:17592554-PrPSc Proteins,
pubmed-meshheading:17592554-Prions
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