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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2007-7-20
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pubmed:abstractText |
Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. Here we systematically assessed the impact of three different total RNA isolation procedures on variation in expression data: method A: lysis of mononuclear cells, followed by lysate homogenization and RNA extraction; method B: organic solvent based RNA isolation, and method C: organic solvent based RNA isolation followed by purification.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-10521349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-10602411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-10676951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-10952317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-11707567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-11707590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-11742071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12075054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12086872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12086890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12105272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12118244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12490681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12664684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12697852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12730115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-12800151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-14603332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15037859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15084693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15155462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15175644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15226186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15238145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15548831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15608262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15609343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15701842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15815718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-15878973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-1596572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-16076888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-16243790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-16761018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-16889801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-16964226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-16964227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-16964228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-16964229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-17061323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-8558195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17587440-9843981
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2164
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
188
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17587440-Analysis of Variance,
pubmed-meshheading:17587440-Gene Expression Profiling,
pubmed-meshheading:17587440-Gene Expression Regulation, Leukemic,
pubmed-meshheading:17587440-Genetic Techniques,
pubmed-meshheading:17587440-Humans,
pubmed-meshheading:17587440-Image Processing, Computer-Assisted,
pubmed-meshheading:17587440-Leukemia,
pubmed-meshheading:17587440-Leukocytes, Mononuclear,
pubmed-meshheading:17587440-Models, Biological,
pubmed-meshheading:17587440-Models, Genetic,
pubmed-meshheading:17587440-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17587440-RNA,
pubmed-meshheading:17587440-RNA, Neoplasm,
pubmed-meshheading:17587440-Solvents,
pubmed-meshheading:17587440-Tumor Markers, Biological
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pubmed:year |
2007
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pubmed:articleTitle |
New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures.
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pubmed:affiliation |
University of Padua, Laboratory of Molecular Diagnostic, Department of Pediatric Oncology, Padua, Italy. marta.campodellorto@unipd.it
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pubmed:publicationType |
Journal Article
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