Source:http://linkedlifedata.com/resource/pubmed/id/17562621
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2007-6-12
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| pubmed:abstractText |
The most frequent chromosomal abnormalities in B-cell chronic lymphocytic leukemia (B-CLL) are deletions on 13q14 and 17p13, trisomy 12, and 14q32 rearrangement. Conventional cytogenetic analysis underestimates the frequency of specific chromosome aberrations in B-CLL because of the low rate of spontaneous mitoses and the poor response to mitogen stimulation. We used interphase fluorescence in situ hybridization (I-FISH) to explore the incidence of chromosomal changes in the peripheral blood cells of B-CLL patients. Probes for 13q14 (D13S319), 17p13 (p53), the centromere of chromosome 12 (CEP12), and 14q32 (IGHC/IGHV) were applied to detect chromosomal aberrations in peripheral blood samples from 83 B-CLL patients (60 men, 23 women). Molecular cytogenetic aberrations were found in 61 cases (73.5%), and 8 patients (9.6%) showed 2 kinds of abnormalities. The most frequent abnormality was deletion of 13q14 (41.0%), followed by +12 (19.3%), deletion of 17p13 (12%), and 14q32 rearrangement (9.6%). FISH results were analyzed for correlation with Binet stages. The percentages of patients who showed abnormalities by FISH were 73.0%, 73.3%, and 80% for Binet stages A, B, and C, respectively, and the percentages of patients with abnormalities who showed 2 anomalies were 7.9%, 27.3%, and 0% for Binet stages A, B, and C, respectively. We noted no consistent pattern among the various Binet stages in the distribution of either the types of FISH-detected anomalies or the numbers of FISH anomalies. I-FISH was found to be a rapid, exact, and sensitive technique for analysis of chromosome aberrations in CLL. FISH could provide accurate information regarding the molecular cytogenetic features of CLL.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
|
| pubmed:issn |
0925-5710
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
85
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
430-6
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:17562621-Adult,
pubmed-meshheading:17562621-Aged,
pubmed-meshheading:17562621-Aged, 80 and over,
pubmed-meshheading:17562621-Asian Continental Ancestry Group,
pubmed-meshheading:17562621-Chromosome Aberrations,
pubmed-meshheading:17562621-Chromosomes, Human, Pair 13,
pubmed-meshheading:17562621-Chromosomes, Human, Pair 17,
pubmed-meshheading:17562621-Female,
pubmed-meshheading:17562621-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:17562621-Humans,
pubmed-meshheading:17562621-Immunoglobulin Heavy Chains,
pubmed-meshheading:17562621-In Situ Hybridization, Fluorescence,
pubmed-meshheading:17562621-Interphase,
pubmed-meshheading:17562621-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:17562621-Male,
pubmed-meshheading:17562621-Middle Aged,
pubmed-meshheading:17562621-Neoplasm Staging
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Interphase fluorescence in situ hybridization detection of cytogenetic abnormalities in B-cell chronic lymphocytic leukemia.
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| pubmed:affiliation |
Department of Hematology, 1st Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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