Source:http://linkedlifedata.com/resource/pubmed/id/17554680
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2007-6-7
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| pubmed:abstractText |
We describe the localization of three proteins of the minichromosome maintenance (MCM) family, Mcm2, -6 and -7 in mouse ovarian oocytes. We showed that Mcm proteins are stored in two forms: soluble and insoluble. Soluble Mcm2, -6 and -7 were uniformly distributed in the nuclei of ovarian oocytes. Insoluble Mcm2 and Mcm7 (but not Mcm6) were detected in the nuclei of resting, growing and fully-grown transcribing oocytes. In transcriptionally inactive fully-grown oocytes, Mcm2 underwent redistribution and Mcm7 disappeared. A similar effect was observed when transcription in growing oocytes was inhibited with alpha-amanitin. We postulate that in mouse oogenesis, the insoluble Mcm proteins are engaged in processes related to regulation of transcription and/or chromatin organization. In oocytes preparing for meiotic maturation, aggregates of the insoluble form of Mcm2 fragmented, dispersed and ultimately disappeared from the nuclei. Numerous Mcm2-positive deposits were observed in the cytoplasm of maturing oocytes. In the one-cell embryo, insoluble Mcm2 appeared in the G1 nucleus, persisted in the S phase and was undetectable in the G2 nucleus. Such behavior of Mcm2 supports its involvement in chromatin licensing in the first embryonic cell cycle.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amanitins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MCM2 protein, mammalian,
http://linkedlifedata.com/resource/pubmed/chemical/Mcm6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Mcm7 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0214-6282
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
51
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
283-95
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:17554680-Amanitins,
pubmed-meshheading:17554680-Animals,
pubmed-meshheading:17554680-Cell Cycle,
pubmed-meshheading:17554680-Cell Cycle Proteins,
pubmed-meshheading:17554680-Crosses, Genetic,
pubmed-meshheading:17554680-DNA-Binding Proteins,
pubmed-meshheading:17554680-Embryo, Mammalian,
pubmed-meshheading:17554680-Female,
pubmed-meshheading:17554680-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:17554680-Immunohistochemistry,
pubmed-meshheading:17554680-Male,
pubmed-meshheading:17554680-Mice,
pubmed-meshheading:17554680-Mice, Inbred C57BL,
pubmed-meshheading:17554680-Mice, Inbred CBA,
pubmed-meshheading:17554680-Nuclear Proteins,
pubmed-meshheading:17554680-Oocytes,
pubmed-meshheading:17554680-Oogenesis,
pubmed-meshheading:17554680-Solubility,
pubmed-meshheading:17554680-Transcription, Genetic
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| pubmed:year |
2007
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| pubmed:articleTitle |
Accumulation and dynamics of proteins of the MCM family during mouse oogenesis and the first embryonic cell cycle.
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| pubmed:affiliation |
Department of Embryology, Institute of Zoology, Warsaw University, Warsaw, Poland.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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