Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-8-6
pubmed:abstractText
To improve efficacy of DNA vaccination, various approaches have been developed, including the use of plasmid expressing co-stimulatory molecules as molecular adjuvants. In this study, we investigated whether co-inoculation of a construct expressing either 4-1BBL or OX40L as the molecular adjuvant with FMDV DNA vaccine, pcD-VP1, can increase immune responses and protective efficacies. Compared to the group immunized with pcD-VP1 alone, the co-inoculation of either molecular adjuvant induced a higher ratio of IgG2a/IgG1, higher levels of expression of IFN-gamma in CD4(+) and CD8(+) T cells and antigen-specific CTL responses, and more importantly provided an enhanced protection against the live FMDV challenge in animals. Concurrently, 4-1BBL as the molecular adjuvant dramatically reduced the viral loads of FMDV in vivo after the challenge. Together, the results demonstrate that co-stimulatory molecules 4-1BBL and OX40L can enhance the antigen-specific cell-mediated responses elicited by VP1 DNA vaccine and provide an enhanced protective efficacy with the reduced viral loads.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0166-3542
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-20
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17540462-4-1BB Ligand, pubmed-meshheading:17540462-Adjuvants, Immunologic, pubmed-meshheading:17540462-Animals, pubmed-meshheading:17540462-Antibodies, Viral, pubmed-meshheading:17540462-CD4-Positive T-Lymphocytes, pubmed-meshheading:17540462-CD8-Positive T-Lymphocytes, pubmed-meshheading:17540462-Cytotoxicity Tests, Immunologic, pubmed-meshheading:17540462-Female, pubmed-meshheading:17540462-Foot-and-Mouth Disease, pubmed-meshheading:17540462-Foot-and-Mouth Disease Virus, pubmed-meshheading:17540462-Guinea Pigs, pubmed-meshheading:17540462-HeLa Cells, pubmed-meshheading:17540462-Humans, pubmed-meshheading:17540462-Immunoglobulin G, pubmed-meshheading:17540462-Injections, Intramuscular, pubmed-meshheading:17540462-Interferon-gamma, pubmed-meshheading:17540462-Mice, pubmed-meshheading:17540462-Mice, Inbred BALB C, pubmed-meshheading:17540462-Neutralization Tests, pubmed-meshheading:17540462-OX40 Ligand, pubmed-meshheading:17540462-Vaccination, pubmed-meshheading:17540462-Vaccines, DNA, pubmed-meshheading:17540462-Viral Load, pubmed-meshheading:17540462-Viral Vaccines
pubmed:year
2007
pubmed:articleTitle
Enhanced protective efficacy and reduced viral load of foot-and-mouth disease DNA vaccine with co-stimulatory molecules as the molecular adjuvants.
pubmed:affiliation
State Key Laboratory for Agro-Biotechnology and the Key Laboratory of Agro-Microbial Resources and Applications of MOA, China Agricultural University, Beijing 100094, China.
pubmed:publicationType
Journal Article