Linked Life Data

17533872

Source:http://linkedlifedata.com/resource/pubmed/id/17533872

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2007-5-30
pubmed:abstractText
Concerns have been raised over the release of C60 nanoparticles into the environment and the potential risk to human health. To address these concerns it is essential to understand the pathways by which nanoparticles enter the cell, where they migrate to, and to establish whether the particles are transformed or modified within the cell. Imaging the subcellular distribution of carbon-based nanoparticles is particularly challenging. It is difficult to achieve high spatial resolution with sufficient image contrast to enable the nanoparticles to be identified within the cell. We have exposed human monocyte-derived macrophages (HMMs) to C60 and used energy filtered transmission electron microscopy (EFTEM) to image the distribution of C60 aggregates within intracellular compartments. We demonstrate that images recorded using low-loss electrons provide a significant improvement in contrast between the cellular material and the C60 allowing a clear differentiation between C60 and unstained cellular compartments and also between ordered and disordered forms of aggregated C60. We confirm that C60 is taken up by HMMs in vitro and is sequestered at several sites within the cell. These sites include the cytoplasm, lysosomes, and most significantly the cell nuclei.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0013-936X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3012-7
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Visualizing the uptake of C60 to the cytoplasm and nucleus of human monocyte-derived macrophage cells using energy-filtered transmission electron microscopy and electron tomography.
pubmed:affiliation
The Nanoscience Centre, University of Cambridge, 11 JJ Thompson Avenue, Cambridge CB3 OFF, UK. aep30@cam.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't