Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2007-7-19
pubmed:abstractText
Mek1 is a meiosis-specific kinase in budding yeast which promotes recombination between homologous chromosomes by suppressing double-strand break (DSB) repair between sister chromatids. Previous work has shown that in the absence of the meiosis-specific recombinase gene, DMC1, cells arrest in prophase due to unrepaired DSBs and that Mek1 kinase activity is required in this situation to prevent repair of the breaks using sister chromatids. This work demonstrates that Mek1 is activated in response to DSBs by autophosphorylation of two conserved threonines, T327 and T331, in the Mek1 activation loop. Using a version of Mek1 that can be conditionally dimerized during meiosis, Mek1 function was shown to be promoted by dimerization, perhaps as a way of enabling autophosphorylation of the activation loop in trans. A putative HOP1-dependent dimerization domain within the C terminus of Mek1 has been identified. Dimerization alone, however, is insufficient for activation, as DSBs and Mek1 recruitment to the meiosis-specific chromosomal core protein Red1 are also necessary. Phosphorylation of S320 in the activation loop inhibits sister chromatid repair specifically in dmc1Delta-arrested cells. Ectopic dimerization of Mek1 bypasses the requirement for S320 phosphorylation, suggesting this phosphorylation is necessary for maintenance of Mek1 dimers during checkpoint-induced arrest.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-10357855, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-10511544, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-10526232, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-10958662, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-10973068, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-11016942, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-11166572, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-11461701, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-11461702, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-11511366, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-11529427, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-11747101, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-12015977, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-12049741, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-12408862, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-12534271, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-12600308, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-1427035, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-14595109, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-14667413, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-14752007, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-15350212, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-15467365, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-1581960, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-15852023, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-16085488, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-16169479, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-16221890, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-16618806, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-16635985, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-16793764, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-16818607, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-17141195, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-17330950, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-7528104, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-7622037, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-8159759, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-8290959, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-8462842, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-8521495, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-8752210, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-8893012, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-9000054, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-9060462, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-9301337, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-9483801, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-9832507, http://linkedlifedata.com/resource/pubmed/commentcorrection/17526735-9880561
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5456-67
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
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