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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1992-1-29
|
| pubmed:abstractText |
The application of DNA typing methods after amplification by the polymerase chain reaction (PCR) of DNA derived from body tissues from charred fire victims was investigated. A total of 26 different tissue specimens from ten extensively burned individuals were analyzed. The samples included femoral muscle, psoas muscle, bone marrow and blood. The post-mortem period varied from 38 to 183 h. After amplifying the DNA by PCR from the various tissues, the D1S80 locus was analyzed with a high resolution polyacrylamide gel electrophoresis technique followed by silver staining and the alleles of the HLA-DQ alpha locus were detected by using a reverse dot blot format. All samples could be typed for both loci and the genotypes were consistent in the various tissues from each individual. A parentage test was performed in two cases and Mendelian inheritance of the alleles for both loci was observed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0379-0738
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
51
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
23-34
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1752592-Alleles,
pubmed-meshheading:1752592-Base Sequence,
pubmed-meshheading:1752592-Bone Marrow,
pubmed-meshheading:1752592-Burns,
pubmed-meshheading:1752592-DNA,
pubmed-meshheading:1752592-DNA Probes,
pubmed-meshheading:1752592-Female,
pubmed-meshheading:1752592-Fires,
pubmed-meshheading:1752592-Forensic Medicine,
pubmed-meshheading:1752592-Genotype,
pubmed-meshheading:1752592-HLA-DQ Antigens,
pubmed-meshheading:1752592-Humans,
pubmed-meshheading:1752592-Male,
pubmed-meshheading:1752592-Molecular Sequence Data,
pubmed-meshheading:1752592-Muscles,
pubmed-meshheading:1752592-Pedigree,
pubmed-meshheading:1752592-Polymerase Chain Reaction,
pubmed-meshheading:1752592-Postmortem Changes,
pubmed-meshheading:1752592-Psoas Muscles
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
The polymerase chain reaction and post-mortem forensic identity testing: application of amplified D1S80 and HLA-DQ alpha loci to the identification of fire victims.
|
| pubmed:affiliation |
Laboratory of Molecular Genetics, National Public Health Institute, Helsinki, Finland.
|
| pubmed:publicationType |
Journal Article
|