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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2007-7-30
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pubmed:abstractText |
Breast cancer development is associated with gene amplification and over expression that is believed to have a causative role in oncogenesis. Previous studies have demonstrated that over expression of TC-1(C8orf4) mRNA occurs in approximately 50% of breast cancer cell lines and primary tumor specimens. Here, we show that TC-1 has transforming properties in human mammary epithelial (HME) cells and its expression is mechanistically linked to FGFR signaling cascades. In vitro experiments demonstrate that TC-1 over expression mediates both anchorage-independent and growth factor-independent proliferation of HME cells. TC-1 was down regulated by the FGFR inhibitor PD173074 in the breast cancer cell line SUM-52 that also has an FGFR2 gene amplification and over expression. Furthermore, forced expression of FGFR2 in HME cells increased the level of expression of endogenous TC-1 mRNA. TC-1 has been implicated as a modulator of Wnt/beta-catenin signaling in 293 cells and in gastric cancer cells. However, while we did find increased expression of a subset of beta-catenin target genes in TC-1 over expressing cells, we did not find an association of TC-1 with global expression of beta-catenin target genes in our cells. Taken together, our data suggest that TC-1 over expression is transforming and may link with the FGFR pathway in a subset of breast cancer.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/C8orf4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/FGFR2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PD 173074,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Fibroblast Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0020-7136
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pubmed:author |
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pubmed:copyrightInfo |
(c) 2007 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
121
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1265-73
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17520678-Animals,
pubmed-meshheading:17520678-Blotting, Northern,
pubmed-meshheading:17520678-Breast Neoplasms,
pubmed-meshheading:17520678-Cell Line, Tumor,
pubmed-meshheading:17520678-Cell Transformation, Neoplastic,
pubmed-meshheading:17520678-Female,
pubmed-meshheading:17520678-Gene Amplification,
pubmed-meshheading:17520678-Gene Expression,
pubmed-meshheading:17520678-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:17520678-Humans,
pubmed-meshheading:17520678-Mice,
pubmed-meshheading:17520678-Neoplasm Proteins,
pubmed-meshheading:17520678-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17520678-Pyrimidines,
pubmed-meshheading:17520678-RNA, Messenger,
pubmed-meshheading:17520678-RNA, Small Interfering,
pubmed-meshheading:17520678-Receptor, Fibroblast Growth Factor, Type 2,
pubmed-meshheading:17520678-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17520678-Signal Transduction,
pubmed-meshheading:17520678-beta Catenin
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pubmed:year |
2007
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pubmed:articleTitle |
Transforming properties of TC-1 in human breast cancer: interaction with FGFR2 and beta-catenin signaling pathways.
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pubmed:affiliation |
Breast Cancer Program, Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201, USA. yangz@karmanos.org
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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