Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-8-3
pubmed:abstractText
The aims of this study were to quantify the change in resting membrane potential (RMP) across the thickness of the circular muscle layer in the mouse and human small intestine and to determine whether the gradient in RMP is dependent on the endogenous production of carbon monoxide (CO). Conventional sharp glass microelectrodes were used to record the RMPs of circular smooth muscle cells at different depths in the human small intestine and in wild-type, HO2-KO, and W/W(V) mutant mouse small intestine. In the wild-type mouse and human intestine, the RMP of circular smooth muscle cells near the myenteric plexus was -65.3 +/- 2 mV and -58.4 +/- 2 mV, respectively, and -60.1 +/- 2 mV and -49.1 +/- 1 mV, respectively, in circular smooth muscle cells at the submucosal border. Oxyhemoglobin (20 microM), a trapping agent for CO, and chromium mesoporphyrin IX, an inhibitor of heme oxygenase, abolished the transwall gradient. The RMP gradients in mouse and human small intestine were not altered by N(G)-nitro-l-arginine (200 microM). No transwall RMP gradient was found in HO2-KO mice and W/W(V) mutant mice. TTX (1 microM) and 1H-[1,2,4-]oxadiazolo[4,3-a]quinoxalin-1-one (10 microM) had no effect on the RMP gradient. These data suggest that the gradient in RMP across the thickness of the circular muscle layer of mouse and human small intestine is CO dependent.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1H-(1,2,4)oxadiazolo(4,3-a)quinoxali..., http://linkedlifedata.com/resource/pubmed/chemical/Carbon Monoxide, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Cyclase, http://linkedlifedata.com/resource/pubmed/chemical/Heme Oxygenase (Decyclizing), http://linkedlifedata.com/resource/pubmed/chemical/Mesoporphyrins, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitroarginine, http://linkedlifedata.com/resource/pubmed/chemical/Oxadiazoles, http://linkedlifedata.com/resource/pubmed/chemical/Oxyhemoglobins, http://linkedlifedata.com/resource/pubmed/chemical/Quinoxalines, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear, http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin, http://linkedlifedata.com/resource/pubmed/chemical/chromium mesoporphyrin, http://linkedlifedata.com/resource/pubmed/chemical/heme oxygenase-2, http://linkedlifedata.com/resource/pubmed/chemical/soluble guanylyl cyclase
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0193-1857
pubmed:author
pubmed:issnType
Print
pubmed:volume
293
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G438-45
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17510199-Animals, pubmed-meshheading:17510199-Carbon Monoxide, pubmed-meshheading:17510199-Enzyme Inhibitors, pubmed-meshheading:17510199-Guanylate Cyclase, pubmed-meshheading:17510199-Heme Oxygenase (Decyclizing), pubmed-meshheading:17510199-Humans, pubmed-meshheading:17510199-Intestine, Small, pubmed-meshheading:17510199-Membrane Potentials, pubmed-meshheading:17510199-Mesoporphyrins, pubmed-meshheading:17510199-Mice, pubmed-meshheading:17510199-Mice, Knockout, pubmed-meshheading:17510199-Mice, Neurologic Mutants, pubmed-meshheading:17510199-Muscle, Smooth, pubmed-meshheading:17510199-Nitric Oxide Synthase, pubmed-meshheading:17510199-Nitroarginine, pubmed-meshheading:17510199-Oxadiazoles, pubmed-meshheading:17510199-Oxyhemoglobins, pubmed-meshheading:17510199-Quinoxalines, pubmed-meshheading:17510199-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:17510199-Tetrodotoxin
pubmed:year
2007
pubmed:articleTitle
Membrane potential gradient is carbon monoxide-dependent in mouse and human small intestine.
pubmed:affiliation
Enteric NeuroScience Program, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, N.I.H., Extramural