Linked Life Data

17500059

Source:http://linkedlifedata.com/resource/pubmed/id/17500059

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
2007-7-2
pubmed:abstractText
Chondroitin sulfate (CS) and dermatan sulfate (DS) have been implicated in the processes of neural development in the brain. In this study, we characterized developmentally regulated brain CS/DS chains using a single chain antibody, GD3G7, produced by the phage display technique. Evaluation of the specificity of GD3G7 toward various glycosaminoglycan preparations showed that this antibody specifically reacted with squid CS-E (rich in the GlcUAbeta1-3GalNAc(4,6-O-sulfate) disaccharide unit E), hagfish CS-H (rich in the IdoUAalpha1-3GalNAc(4,6-O-sulfate) unit iE), and shark skin DS (rich in both E and iE units). In situ hybridization for the expression of N-acetylgalac-tosamine-4-sulfate 6-O-sulfotransferase in the postnatal mouse brain, which is involved in the biosynthesis of CS/DS-E, showed a widespread expression of the transcript in the developing brain except at postnatal day 7, where strong expression was observed in the external granule cell layer in the cerebellum. The expression switched from the external to internal granule cell layer with development. Immunohistochemical localization of GD3G7 in the mouse brain showed that the epitope was relatively abundant in the cerebellum, hippocampus, and olfactory bulb. GD3G7 suppressed the growth of neurites in embryonic hippocampal neurons mediated by CS-E, suggesting that the epitope is embedded in the neurite outgrowth-promoting motif of CS-E. In addition, a CS-E decasaccharide fraction was found to be the critical minimal structure needed for recognition by GD3G7. Four discrete decasaccharide epitopic sequences were identified. The antibody GD3G7 has broad applications in investigations of CS/DS chains during the central nervous system's development and under various pathological conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
282
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19442-52
pubmed:meshHeading
pubmed-meshheading:17500059-Animals, pubmed-meshheading:17500059-Animals, Newborn, pubmed-meshheading:17500059-Antibodies, Monoclonal, pubmed-meshheading:17500059-Antibody Specificity, pubmed-meshheading:17500059-Brain, pubmed-meshheading:17500059-Brain Chemistry, pubmed-meshheading:17500059-Chondroitin Sulfates, pubmed-meshheading:17500059-Dermatan Sulfate, pubmed-meshheading:17500059-Disaccharides, pubmed-meshheading:17500059-Epitopes, pubmed-meshheading:17500059-Female, pubmed-meshheading:17500059-Gene Expression Regulation, Developmental, pubmed-meshheading:17500059-Gene Expression Regulation, Enzymologic, pubmed-meshheading:17500059-Immunoglobulin Variable Region, pubmed-meshheading:17500059-Immunohistochemistry, pubmed-meshheading:17500059-Mice, pubmed-meshheading:17500059-Neurites, pubmed-meshheading:17500059-Pregnancy, pubmed-meshheading:17500059-Rats, pubmed-meshheading:17500059-Sulfotransferases
pubmed:year
2007
pubmed:articleTitle
Functions of chondroitin sulfate/dermatan sulfate chains in brain development. Critical roles of E and iE disaccharide units recognized by a single chain antibody GD3G7.
pubmed:affiliation
Department of Biochemistry, Kobe Pharmaceutical University, Higashinada-ku, Kobe 658-8558, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't