|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0162713,
umls-concept:C0206333,
umls-concept:C0220781,
umls-concept:C0243071,
umls-concept:C0443220,
umls-concept:C1167622,
umls-concept:C1510827,
umls-concept:C1513371,
umls-concept:C1704241,
umls-concept:C1883254,
umls-concept:C1959007,
umls-concept:C2603343
|
|
pubmed:issue |
13
|
|
pubmed:dateCreated |
2007-5-28
|
|
pubmed:abstractText |
A series of thio- and selenopyrylium analogues of 2,4-di(4-dimethylaminophen-yl)-6-methylthiopyrylium iodide were prepared in five steps from 4-dimethylaminophenyl-propargyl aldehyde and the corresponding lithium acetylide. When bound to DNA, all of the dyes absorb at wavelengths >600nm, which avoids the hemoglobin band I maximum at 575nm. The binding of the series of dyes to double-stranded DNA was examined spectrophotometrically and by isothermal titration calorimetry to determine binding constants, by a topoisomerase I DNA unwinding assay, by competition dialysis with [poly(dGdC)](2) and [poly(dAdT)](2), and by ethidium bromide displacement studies to examine propensities for intercalation, and by circular dichroism studies. The dyes were found to show mixed binding modes.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0968-0896
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
15
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4406-18
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:17498962-Binding, Competitive,
pubmed-meshheading:17498962-Blood-Borne Pathogens,
pubmed-meshheading:17498962-Calorimetry,
pubmed-meshheading:17498962-Chemistry, Physical,
pubmed-meshheading:17498962-Circular Dichroism,
pubmed-meshheading:17498962-DNA, Bacterial,
pubmed-meshheading:17498962-DNA, Superhelical,
pubmed-meshheading:17498962-Dialysis,
pubmed-meshheading:17498962-Enzyme Inhibitors,
pubmed-meshheading:17498962-Ethidium,
pubmed-meshheading:17498962-Indicators and Reagents,
pubmed-meshheading:17498962-Magnetic Resonance Spectroscopy,
pubmed-meshheading:17498962-Nucleic Acid Conformation,
pubmed-meshheading:17498962-Photosensitizing Agents,
pubmed-meshheading:17498962-Physicochemical Phenomena,
pubmed-meshheading:17498962-Spectrophotometry, Ultraviolet,
pubmed-meshheading:17498962-Thiophenes,
pubmed-meshheading:17498962-Topoisomerase I Inhibitors
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Synthesis of analogues of a flexible thiopyrylium photosensitizer for purging blood-borne pathogens and binding mode and affinity studies of their complexes with DNA.
|
|
pubmed:affiliation |
Department of Chemistry, State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA. mcknight@geneseo.edu
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
