Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-7-20
pubmed:abstractText
The human hydroxysteroid sulfotransferase (SULT) 2B1 gene is a member of the cytosolic SULT gene superfamily. The two SULT2B1 isoforms, SULT2B1a and SULT2B1b, are encoded by a single gene as a result of alternative transcription initiation and alternative splicing. SULT2B1b catalyzes the sulfonation of 3beta-hydroxysteroid hormones and cholesterol, whereas SULT2B1a preferentially catalyzes pregnenolone sulfonation. We used a genotype-to-phenotype approach to identify and characterize common sequence variation in SULT2B1. Specifically, we resequenced all exons, splice junctions, and approximately 2.5 kb of the 5'-flanking regions (FRs) for each isoform using 60 DNA samples each from African-American and Caucasian-American subjects. We observed 100 polymorphisms, including four nonsynonymous coding single nucleotide polymorphisms and one 6-base pair deletion-all within the "shared" region of the open reading frame. Functional genomic studies of the wild type (WT) and five variant allozymes for each isoform performed with a mammalian expression system showed that variant allozyme activities ranged from 64 to 88% of WT for SULT2B1a and from 76 to 98% for SULT2B1b. Relative levels of immunoreactive protein were similar to those for enzyme activity. Luciferase reporter gene constructs for 2.5 kb of the SULT2B1b 5'-FR displayed a cell line-dependent pattern of variation in activity. Finally, deletion of the proline-rich SULT2B1 carboxyl terminus resulted in intracellular protein aggregate formation and accelerated degradation of the truncated protein. These studies resulted in the identification of common SULT2B1 gene sequence variation, as well as insight into the effects of that variation on the function of this important steroid-metabolizing enzyme.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-3565
pubmed:author
pubmed:issnType
Print
pubmed:volume
322
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
529-40
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17496163-5' Flanking Region, pubmed-meshheading:17496163-African Americans, pubmed-meshheading:17496163-Animals, pubmed-meshheading:17496163-COS Cells, pubmed-meshheading:17496163-Cell Line, Tumor, pubmed-meshheading:17496163-Cercopithecus aethiops, pubmed-meshheading:17496163-Cysteine Proteinase Inhibitors, pubmed-meshheading:17496163-Cytosol, pubmed-meshheading:17496163-European Continental Ancestry Group, pubmed-meshheading:17496163-Gene Deletion, pubmed-meshheading:17496163-Gene Expression, pubmed-meshheading:17496163-Gene Frequency, pubmed-meshheading:17496163-Green Fluorescent Proteins, pubmed-meshheading:17496163-Haplotypes, pubmed-meshheading:17496163-Humans, pubmed-meshheading:17496163-Isoenzymes, pubmed-meshheading:17496163-Leupeptins, pubmed-meshheading:17496163-Linkage Disequilibrium, pubmed-meshheading:17496163-Microscopy, Confocal, pubmed-meshheading:17496163-Mutation, pubmed-meshheading:17496163-Pharmacogenetics, pubmed-meshheading:17496163-Polymorphism, Genetic, pubmed-meshheading:17496163-Recombinant Fusion Proteins, pubmed-meshheading:17496163-Sequence Analysis, DNA, pubmed-meshheading:17496163-Sulfotransferases
pubmed:year
2007
pubmed:articleTitle
Human hydroxysteroid sulfotransferase SULT2B1 pharmacogenomics: gene sequence variation and functional genomics.
pubmed:affiliation
Division of Clinical Pharmacology, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural