Linked Life Data

17495465

Source:http://linkedlifedata.com/resource/pubmed/id/17495465

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2007-5-14
pubmed:abstractText
Cell migration is crucial for tumor metastasis. Membrane ion channels may play a major role in tumor cell migration because the cells must undergo changes in shape and volume during migration. In the present study, we used the transwell migration assay, an in vitro model for cell migration, and the patch-clamp technique to investigate the role of the volume-activated Cl(-) current (I(cl,vol)) in the regulation of the migration of nasopharyngeal carcinoma CNE-2Z cells. 5-Nitro-2- (3-phenylpropylamino) benzoic acid (NPPB) inhibited the I(cl,vol) and the migration of CNE-2Z cells with almost identical dose-dependent pattern (IC(50) of 98.1 microM and 97.7 microM for I(cl,vol) and cell migration, respectively). Extracellular adenosine triphosphate (ATP) also showed similar dose-dependent inhibitory effects on the currents and migration (IC(50) of 1.07mM, and 1.11mM for I(cl,vol) and cell migration, respectively). Hypotonic treatments, which activated I(cl,vol), increased cell migration. Exposure to hypertonic solutions, which was shown to suppress I(cl,vol), inhibited cell migration. Replacement of Cl(-) with gluconate, which is relatively chloride channel-impermeable, impaired cell migration, whereas substitution of Cl(-) by I(-) and Br(-), the chloride channel-permeable ions, did not significantly affect cell migration. Analysis of the effects of all the above treatments on I(cl,vol) and cell migration indicated that the inhibition of migration was positively correlated with the blockage of I(cl,vol), with a correlation coefficient (r) of 0.97, suggesting a functional relationship between I(cl,vol) and cell migration. These data suggest that the volume-activated Cl(-) channels are involved in cell migration.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1015-8987
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2007 S. Karger AG, Basel.
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
249-58
pubmed:dateRevised
2007-8-13
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Blockage of volume-activated chloride channels inhibits migration of nasopharyngeal carcinoma cells.
pubmed:affiliation
Institute of Basic Medical Sciences, Guangdong Pharmaceutical University, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't