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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-1-21
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pubmed:abstractText |
Previous experiments have established the presence of a 30-kD DNA binding protein on the surface of human leukocytes. Herein we report that selected sera from patients with systemic lupus erythematosus (SLE) and MCTD are reactive with a 28-30 kD protein on immunoblots of peripheral blood mononuclear cells (PBMC) cell membrane preparations; the reactivity is abolished by prior incubation of the blot with DNA. Antibodies eluted from the 28-30 kD strip inhibited the binding of 3H. DNA to human PBMC. An immunomatrix of 28-30 kD reactive immunoglobulins was able to extract a 29-kD DNA binding protein from a PBMC cell membrane preparation. Flow cytometry experiments confirmed the cell surface IgG reactivity of sera with T lymphocytes. Additional experiments indicated that cell surface IgG binding was not due to antibodies binding to cell surface DNA, DNA anti-DNA immune complexes reacting with a DNA binding protein, anti-histone antibodies or anti-Sm antibodies. It is hypothesized that this autoimmune response could be one component of an idiotypic network involving anti-DNA antibodies.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-1081876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-1689348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2217546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2332503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2365827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2452195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2660143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2706026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2781137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2821153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-2868209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-3001145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-315291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-3490571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-3492514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-3497339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-3606686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-375379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-3876357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-4142565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6153898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6182766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6210711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6211490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6231307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6236803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6374668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6600174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-6985634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-7138600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1747945-7414333
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
374-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1747945-Autoantibodies,
pubmed-meshheading:1747945-Autoimmunity,
pubmed-meshheading:1747945-Blotting, Western,
pubmed-meshheading:1747945-Cell Membrane,
pubmed-meshheading:1747945-DNA-Binding Proteins,
pubmed-meshheading:1747945-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1747945-Flow Cytometry,
pubmed-meshheading:1747945-Humans,
pubmed-meshheading:1747945-Lupus Erythematosus, Systemic,
pubmed-meshheading:1747945-Mixed Connective Tissue Disease,
pubmed-meshheading:1747945-T-Lymphocytes
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pubmed:year |
1991
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pubmed:articleTitle |
Autoimmunity to a 28-30 kD cell membrane DNA binding protein: occurrence in selected sera from patients with SLE and mixed connective tissue disease (MCTD).
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pubmed:affiliation |
Department of Medicine, Oregon Health Sciences University, Portland 97201.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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