| pubmed-article:17467221 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17467221 | lifeskim:mentions | umls-concept:C0205474 | lld:lifeskim |
| pubmed-article:17467221 | lifeskim:mentions | umls-concept:C0001699 | lld:lifeskim |
| pubmed-article:17467221 | lifeskim:mentions | umls-concept:C0597979 | lld:lifeskim |
| pubmed-article:17467221 | lifeskim:mentions | umls-concept:C0314603 | lld:lifeskim |
| pubmed-article:17467221 | lifeskim:mentions | umls-concept:C0456387 | lld:lifeskim |
| pubmed-article:17467221 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:17467221 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:17467221 | pubmed:dateCreated | 2007-8-10 | lld:pubmed |
| pubmed-article:17467221 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467221 | pubmed:abstractText | The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 beta-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5-aac(6')-IIa-blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most beta-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4. | lld:pubmed |
| pubmed-article:17467221 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17467221 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467221 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17467221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17467221 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17467221 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:17467221 | pubmed:issn | 0732-8893 | lld:pubmed |
| pubmed-article:17467221 | pubmed:author | pubmed-author:LeeSang HeeSH | lld:pubmed |
| pubmed-article:17467221 | pubmed:author | pubmed-author:JeongSeok... | lld:pubmed |
| pubmed-article:17467221 | pubmed:author | pubmed-author:LeeJung HunJH | lld:pubmed |
| pubmed-article:17467221 | pubmed:author | pubmed-author:BaeIl KwonIK | lld:pubmed |
| pubmed-article:17467221 | pubmed:author | pubmed-author:YounHasikH | lld:pubmed |
| pubmed-article:17467221 | pubmed:author | pubmed-author:HongSeong... | lld:pubmed |
| pubmed-article:17467221 | pubmed:author | pubmed-author:KimHyoung... | lld:pubmed |
| pubmed-article:17467221 | pubmed:author | pubmed-author:LeeYou-NaeYN | lld:pubmed |
| pubmed-article:17467221 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17467221 | pubmed:volume | 58 | lld:pubmed |
| pubmed-article:17467221 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17467221 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17467221 | pubmed:pagination | 465-8 | lld:pubmed |
| pubmed-article:17467221 | pubmed:meshHeading | pubmed-meshheading:17467221... | lld:pubmed |
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| pubmed-article:17467221 | pubmed:meshHeading | pubmed-meshheading:17467221... | lld:pubmed |
| pubmed-article:17467221 | pubmed:meshHeading | pubmed-meshheading:17467221... | lld:pubmed |
| pubmed-article:17467221 | pubmed:meshHeading | pubmed-meshheading:17467221... | lld:pubmed |
| pubmed-article:17467221 | pubmed:meshHeading | pubmed-meshheading:17467221... | lld:pubmed |
| pubmed-article:17467221 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17467221 | pubmed:articleTitle | Genetic and biochemical characterization of GES-5, an extended-spectrum class A beta-lactamase from Klebsiella pneumoniae. | lld:pubmed |
| pubmed-article:17467221 | pubmed:affiliation | Research Institute for Antimicrobial Resistance, Department of Laboratory Medicine, Kosin University College of Medicine, 602-030, 34 Amnam-Dong, Suh-Gu, Busan, South Korea. | lld:pubmed |
| pubmed-article:17467221 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17467221 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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