Linked Life Data

17467221

Source:http://linkedlifedata.com/resource/pubmed/id/17467221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-8-10
pubmed:databankReference
pubmed:abstractText
The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 beta-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5-aac(6')-IIa-blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most beta-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0732-8893
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-8
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Genetic and biochemical characterization of GES-5, an extended-spectrum class A beta-lactamase from Klebsiella pneumoniae.
pubmed:affiliation
Research Institute for Antimicrobial Resistance, Department of Laboratory Medicine, Kosin University College of Medicine, 602-030, 34 Amnam-Dong, Suh-Gu, Busan, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't