Linked Life Data

17452091

Source:http://linkedlifedata.com/resource/pubmed/id/17452091

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-5-14
pubmed:databankReference
pubmed:abstractText
The O-antigen gene clusters of Escherichia coli serogroups O117, O126, and O146 were sequenced, and 11, 10, and 11 open reading frames (ORFs) were identified, respectively. Genes required for O-antigen sugar biosynthesis, sugar transfer, and sugar processing were identified. Multiplex polymerase chain reaction (PCR) assays were developed targeting the wzx and wzy genes present in the O-antigen gene cluster of these serogroups. The assays were highly serogroup specific when tested against strains belonging to serogroups that were isolated from food, humans, animals, and environmental sources, as well as against representative strains belonging to ca. 165 different E. coli O serogroups and a number of non-E. coli bacteria. Thus, the results demonstrate that the wzx and wzy gene sequences were specific to E. coli O117, O126, and O146 and can be used as diagnostic markers for rapid identification and detection of these serogroups.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0890-8508
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
295-302
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Sequencing and analysis of the Escherichia coli serogroup O117, O126, and O146 O-antigen gene clusters and development of PCR assays targeting serogroup O117-, O126-, and O146-specific DNA sequences.
pubmed:affiliation
Eastern Regional Research Center, US Department of Agriculture, Agricultural Research Service, Wyndmoor, PA 19038, USA.
pubmed:publicationType
Journal Article