Linked Life Data

17450119

Source:http://linkedlifedata.com/resource/pubmed/id/17450119

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-4-23
pubmed:abstractText
Intestinal cells are the first line of defense against toxic substances that may enter the body orally. Some of these substances may emanate from wastewater treatment plants and thus may eventually enter the food chain. In this study, human intestinal Caco-2 cells were incubated with biologically treated activated sludge and lipopolysaccharide (LPS) to determine changes in protein expression and cell monolayer integrity. Caco-2 cells incubated for 3 h with 24-h-aerated activated sludge or 220 microg/ml LPS showed enhanced expression of the heat shock protein 90b (Hsp90b). The results further show that 220 microg/ml LPS is sufficient to irreversibly disrupt tight-junction permeability in 30 min. The Hsp90b expression by the Caco-2 cells incubated with biologically treated activated sludge may be a cellular protective mechanism against LPS-induced stress.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0915-955X
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-9
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Human intestinal cells incubated with activated sludge and lipopolysaccharide express Hsp90b.
pubmed:affiliation
Department of Environmental Engineering, Graduate School of Engineering, Hokkaido University, Kita-13, Nishi-8, Sapporo, Hokkaido, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't