Source:http://linkedlifedata.com/resource/pubmed/id/17440958
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2007-11-26
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| pubmed:abstractText |
The purpose of the present study is to establish and characterize a conditionally immortalized astrocyte cell line and to clarify the genetic networks responsible for the cell growth arrest and differentiation. A conditionally immortalized astrocyte cell line, RCG-12, was established by infecting primary cultured rat cortical glia cells with a temperature-sensitive simian virus 40 large T-antigen. At a permissive temperature of 33 degrees C, the large T-antigen was expressed and cells grew continuously. On the other hand, the down-regulation of T-antigen at a non-permissive temperature of 39 degrees C led to growth arrest and differentiation. The cells expressed astrocyte-expressed genes such as glial fibrillary acidic protein. Interestingly, the differentiated condition induced by the non-permissive temperature significantly elevated the expression levels of several astrocyte-expressed genes. To identify the detailed mechanisms by which non-permissive temperature-induced cell growth arrest and differentiation, we performed high-density oligonucleotide microarray analysis and found that 556 out of 15,923 probe sets were differentially expressed 2.0-fold. A computational gene network analysis revealed that a genetic network containing up-regulated genes such as RB, NOTCH1, and CDKN1A was associated with the cellular growth and proliferation, and that a genetic network containing down-regulated genes such as MYC, CCNB1, and IGF1 was associated with the cell cycle. The established cell line RCG-12 retains some characteristics of astrocytes and should provide an excellent model for studies of astrocyte biology. The present results will also provide a basis for understanding the detailed molecular mechanisms of the growth arrest and differentiation of astrocytes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1a protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0730-2312
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright (c) 2007 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
102
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1472-85
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| pubmed:meshHeading |
pubmed-meshheading:17440958-Animals,
pubmed-meshheading:17440958-Antigens, Polyomavirus Transforming,
pubmed-meshheading:17440958-Astrocytes,
pubmed-meshheading:17440958-Cell Count,
pubmed-meshheading:17440958-Cell Differentiation,
pubmed-meshheading:17440958-Cell Line, Transformed,
pubmed-meshheading:17440958-Cell Proliferation,
pubmed-meshheading:17440958-Cell Transformation, Viral,
pubmed-meshheading:17440958-Cerebral Cortex,
pubmed-meshheading:17440958-Computational Biology,
pubmed-meshheading:17440958-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:17440958-Embryo, Mammalian,
pubmed-meshheading:17440958-Gene Expression Regulation,
pubmed-meshheading:17440958-Gene Regulatory Networks,
pubmed-meshheading:17440958-Glial Fibrillary Acidic Protein,
pubmed-meshheading:17440958-Immunohistochemistry,
pubmed-meshheading:17440958-Models, Genetic,
pubmed-meshheading:17440958-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17440958-Rats,
pubmed-meshheading:17440958-Rats, Sprague-Dawley,
pubmed-meshheading:17440958-Temperature,
pubmed-meshheading:17440958-Transfection
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| pubmed:year |
2007
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| pubmed:articleTitle |
Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12.
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| pubmed:affiliation |
Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan. takasaki@cts.u-toyama.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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