Linked Life Data

17434448

Source:http://linkedlifedata.com/resource/pubmed/id/17434448

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-4-25
pubmed:abstractText
An osteoblast-specific transcription factor, core binding factor alpha1 (Cbfalpha1), is critical for osteoblast and odontoblast differentiation. In this study, the role of Cbfalpha1 in human dentin matrix protein1 (DMP1) gene expression in human dental pulp stem cells (HDPSCs) was investigated. The desired promoter fragments were obtained and cloned into the pGL3-basic vector. It was found that Cbfalpha1 isoforms were predominantly expressed in the cytoplasm of the HDPSCs and reached to the maximum after transfection for 48h. Furthermore, forced overexpression of Cbfalpha1 induced the increase of the luciferase activities of pGL3-P1-6, especially those of pGL3-P(-505to+86) (p<0.05) were the most significant. Then the site-directed mutagenesis of Cbfalpha1 binding sites in the promoter region of nt -505 to +86 resulted in a marked decline of luciferase activities. Thus, our results suggest that Cbfalpha1 upregulates DMP1 gene expression differentially that may contribute to the spatial-temporal expression pattern of DMP1 during odontoblast differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
357
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
505-10
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Upregulation of dentin matrix protein 1 promoter activities by core binding factor alpha1 in human dental pulp stem cells.
pubmed:affiliation
Department of Stomatology, The General Hospital of the Air Force P.L.A., Beijing 100036, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't