Linked Life Data

17416379

Source:http://linkedlifedata.com/resource/pubmed/id/17416379

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2007-6-5
pubmed:abstractText
A new, simple and sensitive high-performance liquid chromatography (HPLC) method with fluorescence detection was developed and validated for the determination of vardenafil in small volumes of rat plasma and bile. The absorbance and fluorescence characteristics of vardenafil were studied and factors that affect the HPLC resolution and fluorescence intensity were examined and optimized. Vardenafil and the internal standard cisapride were extracted using acetonitrile. The separation was achieved on a C18 column at 35 degrees C using acetonitrile-50 mM ammonium acetate aqueous solution (pH 6.8) (40:60) as mobile phase. At a flow rate of 1 ml/min, the total run time was 18 min. Fluorescence was measured with excitation and emission set at 280 and 470 nm, respectively. The calibration curves were linear from 10 to 1000 ng/ml and 0.2-100 microg/ml for plasma and bile samples, respectively. The intra- and inter-day imprecision did not exceed 10.8%, and the accuracy was within 9.6% deviation of the nominal concentration. The method was used successfully to investigate the disposition and biliary excretion of vardenafil in rats.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
1154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
222-9
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Development and validation of a high-performance liquid chromatographic method using fluorescence detection for the determination of vardenafil in small volumes of rat plasma and bile.
pubmed:affiliation
Department of Pharmacy, Chia-Nan University of Pharmacy and Science, No. 6, Section 1, Erh-Jen Road, Tainan 71710, Taiwan. hccl@mail.chna.edu.tw
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies