Linked Life Data

17386594

Source:http://linkedlifedata.com/resource/pubmed/id/17386594

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-3-27
pubmed:abstractText
An unmodified gold nanoparticle-based colorimetric assay system in homogeneous format has been developed using hydrogen peroxide (H(2)O(2)) as a model analyte. H(2)O(2) is added to o-phenylenediamine/horseradish peroxidase solution, and allowed to react for 10 min. Then, unmodified gold nanoparticles that serve as "reaction indicators" are added to the reaction solution. The resulting mixture color changes dramatically from red to blue. The reason is that azoaniline, a horseradish peroxidase-catalyzed oxidation product, induces the nanoparticle aggregation. Using this approach, H(2)O(2) can be semiquantitatively determined over the concentration range of approximately 4 orders of magnitude by the naked eye. If the observed peak intensity at 420 nm is used for the construction of the calibration plot, hydrogen peroxide can be accurately determined down to concentration levels of 1.3 x 10(-6) M. Compared with the conventional electrochemical protocol, this sensing system offers several important advantages: (1) ability to be monitored by the naked eye, (2) avoiding the need of surface modification of electrodes or gold nanoparticles and (3) detection in homogeneous solution. It is worthy of note that this efficient and convenient strategy is also suitable for the detection of other species, such as glucose and cholesterol.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-4324
pubmed:author
pubmed:issnType
Electronic
pubmed:day
12
pubmed:volume
584
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
122-8
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Homogeneous, unmodified gold nanoparticle-based colorimetric assay of hydrogen peroxide.
pubmed:affiliation
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't