Linked Life Data

17385045

Source:http://linkedlifedata.com/resource/pubmed/id/17385045

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2007-6-20
pubmed:abstractText
Cell mechanical properties are important in the adhesion of endothelial cells to synthetic vascular grafts exposed to shear flow. We hypothesized that the local apparent elastic modulus of the nucleus and the cell body would increase to a greater extent for cells adherent via the dual ligand (integrin-fibronectin/avidin-biotin) and exposed to flow, than for cells treated with either ligand alone. High affinity avidin-biotin bonds and in vitro flow exposure were used to improve adhesion to grafts thereby altering the mechanical properties of endothelial cells. Introduction of the dual ligand chemistry at the cell-substrate interface increased the apparent elastic modulus of the cells as compared to cells adherent with the fibronectin-integrin bonds only. Cells cultured on the dual ligand surface exhibited higher elastic moduli of the nucleus and cell body relative to cells cultured on fibronectin alone. Exposure of cells to flow increased the apparent elastic modulus of the cell body, nucleus, and stress fibers of cells adherent to the fibronectin surface. A similar effect was seen for cells adherent to the dual ligand surface, although there was little effect on the elastic modulus of the nucleus. While the dual ligand surface produces an increase in adhesion strength, focal contact area and elastic modulus, the change in elastic modulus after exposure to flow is due only to an increase in stress fibers and not an increase in contact area.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0090-6964
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1120-30
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Flow and high affinity binding affect the elastic modulus of the nucleus, cell body and the stress fibers of endothelial cells.
pubmed:affiliation
Department of Biomedical Engineering, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural