Source:http://linkedlifedata.com/resource/pubmed/id/17373089
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2007-3-20
|
| pubmed:abstractText |
Typeability, reproducibility, and discriminatory power of ERIC-PCR and Apal-PFGE to establish the genetic relation of P. multocida strains were determined. Forty-nine strains of different source, biotype, capsular group, somatic serotype, and resistance to antimicrobials were studied. By ERIC-PCR, 31 patterns were defined with 10 to 14 bands in a rank of 0.2 and 1.2 kb. By Apal-PFGE, 37 restriction patterns were established with 7 to 15 bands of 34 to 450 kb. Typeability was 100% (T=1) for ERIC-PCR, and 94% (T = 0.94) for Apal-PFGE. Reproducibility of both techniques was 100% (R=1). Discriminatory power was 93% (D = 0.93) for ERIC-PCR, and 98% (D = 0.98) for Apal-PFGE. By using both techniques, epidemiologically related strains were grouped, and unrelated strains were clearly differentiated. The value of ERIC-PCR and Apal-PFGE as complements to epidemiologic studies was demonstrated, especially when both techniques were used to analyze the strains.
|
| pubmed:language |
spa
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0325-7541
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
190-6
|
| pubmed:dateRevised |
2008-8-28
|
| pubmed:meshHeading |
pubmed-meshheading:17373089-Americas,
pubmed-meshheading:17373089-Animals,
pubmed-meshheading:17373089-Antarctic Regions,
pubmed-meshheading:17373089-Australia,
pubmed-meshheading:17373089-Bird Diseases,
pubmed-meshheading:17373089-Birds,
pubmed-meshheading:17373089-Cattle,
pubmed-meshheading:17373089-Cattle Diseases,
pubmed-meshheading:17373089-Chickens,
pubmed-meshheading:17373089-DNA, Bacterial,
pubmed-meshheading:17373089-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:17373089-Drug Resistance, Bacterial,
pubmed-meshheading:17373089-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:17373089-Humans,
pubmed-meshheading:17373089-Pasteurella Infections,
pubmed-meshheading:17373089-Pasteurella multocida,
pubmed-meshheading:17373089-Polymerase Chain Reaction,
pubmed-meshheading:17373089-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:17373089-Poultry Diseases,
pubmed-meshheading:17373089-Reproducibility of Results,
pubmed-meshheading:17373089-Swine,
pubmed-meshheading:17373089-Swine Diseases,
pubmed-meshheading:17373089-Turkeys
|
| pubmed:articleTitle |
[Evaluation of two techniques of molecular subtyping to study Pasteurella multocida].
|
| pubmed:affiliation |
Servicio Fisiopatogenia,Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas--Administracidó Nacional de Laboratorios e Institutos de Salud Dr. Carlos G. Malbrin, Buenos Aires, Argentina. galeotta@anlis.gov.ar
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract,
Evaluation Studies
|