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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-4-5
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pubmed:abstractText |
Enhanced expression of valosin-containing protein (VCP) correlates with invasion and metastasis of cancers. To clarify the transcription mechanism of VCP, human and mouse genomic sequence was compared, revealing a 260 bp DNA sequence in the 5'-flanking region of VCP gene to be highly conserved between the two, in which binding motif of E74-like factor 2/new Ets-related factor (ELF2/NERF) was identified. Chromatin immunoprecipitation assay showed binding of ELF2/NERF to the 5'-flanking region of VCP gene. Knock-down of ELF2/NERF by siRNA decreased expression level of VCP. Viability of cells under tumor necrosis factor-alpha treatment significantly reduced in ELF2/NERF-knock-down breast cancer cell line. Immunohistochemical analysis on clinical breast cancer specimens showed a correlation of nuclear ELF2/NERF expression with VCP expression and proliferative activity of cells shown by Ki-67 immunohistochemistry. These findings indicate that ELF2/NERF promotes VCP transcription and that ELF2/NERF-VCP pathway might be important for cell survival and proliferation under cytokine stress.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
356
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
536-41
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pubmed:dateRevised |
2009-9-3
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pubmed:meshHeading |
pubmed-meshheading:17368566-5' Flanking Region,
pubmed-meshheading:17368566-Adenosine Triphosphatases,
pubmed-meshheading:17368566-Adult,
pubmed-meshheading:17368566-Aged,
pubmed-meshheading:17368566-Aged, 80 and over,
pubmed-meshheading:17368566-Animals,
pubmed-meshheading:17368566-Apoptosis,
pubmed-meshheading:17368566-Base Sequence,
pubmed-meshheading:17368566-Breast Neoplasms,
pubmed-meshheading:17368566-Carcinoma,
pubmed-meshheading:17368566-Cell Cycle Proteins,
pubmed-meshheading:17368566-Cell Line, Tumor,
pubmed-meshheading:17368566-Cell Proliferation,
pubmed-meshheading:17368566-Chromatin Immunoprecipitation,
pubmed-meshheading:17368566-Female,
pubmed-meshheading:17368566-Gene Expression Regulation,
pubmed-meshheading:17368566-Humans,
pubmed-meshheading:17368566-Immunohistochemistry,
pubmed-meshheading:17368566-Ki-67 Antigen,
pubmed-meshheading:17368566-Mice,
pubmed-meshheading:17368566-Middle Aged,
pubmed-meshheading:17368566-Molecular Sequence Data,
pubmed-meshheading:17368566-RNA Interference,
pubmed-meshheading:17368566-Sequence Alignment,
pubmed-meshheading:17368566-Transcription Factors
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pubmed:year |
2007
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pubmed:articleTitle |
E74-like factor 2 regulates valosin-containing protein expression.
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pubmed:affiliation |
Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
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pubmed:publicationType |
Journal Article
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