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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-3-19
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pubmed:abstractText |
A simple and rapid method for preparation of plasmid DNA from overnight incubation was introduced. It does not require any additional reagents; the incubation mixture containing recombinant plasmid DNA was just mixed with H2O and phenol/chloroform/isoamyl alcohol in certain ratio. After vortexing and spinning of the mixture, the supernatant could be directly loaded onto agarose gel and analyzed using electrophoresis. The whole preparation requires only 3-5 minutes. So to quickly screen recombinant clones, this method is better compared with traditional methods.
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pubmed:language |
chi
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1000-3061
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
176-8
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pubmed:meshHeading |
pubmed-meshheading:17366910-Centrifugation, Density Gradient,
pubmed-meshheading:17366910-Chloroform,
pubmed-meshheading:17366910-Cloning, Molecular,
pubmed-meshheading:17366910-DNA, Bacterial,
pubmed-meshheading:17366910-Deoxyribonuclease HindIII,
pubmed-meshheading:17366910-Electrophoresis,
pubmed-meshheading:17366910-Escherichia coli,
pubmed-meshheading:17366910-Pentanols,
pubmed-meshheading:17366910-Phenol,
pubmed-meshheading:17366910-Plasmids,
pubmed-meshheading:17366910-Reproducibility of Results,
pubmed-meshheading:17366910-Time Factors,
pubmed-meshheading:17366910-Water
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pubmed:year |
2007
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pubmed:articleTitle |
[A rapid method for preparation of plasmid DNA for screening recombinant clones].
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pubmed:affiliation |
The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China.
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pubmed:publicationType |
Journal Article,
English Abstract
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