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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2007-4-16
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pubmed:abstractText |
The water channel, Aquaporin-9 (AQP9) is enriched in selected neuronal populations and is unique its ability to act as a lactate-glycerol channel supplying neurons with alternative fuel under ischaemic conditions. AQP9 was detected in RGC-5 cells, a retinal ganglion cell-line, primary RGCs, and retina by Western blotting, real-time PCR (RT-PCR) and immunohistochemistry. RGC-5 cells subjected to a hypotonic stress increased their cell volume that was blocked by known inhibitor of AQP9 (phloretin (40 microM)). RGC-5 cells subjected to hypoxia, showed an up-regulation in AQP9 expression as judged by Western blotting and RT-PCR. Similarly, hypotonic shock (50%) increased AQP9 expression as determined by RT-PCR. AQP9 is involved in energy balance as a glycerol-lactate channel and also appears to regulate cell volume in retinal ganglion neurons. This water channel may play a key role in retinal ganglion pathology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1043-6618
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
378-84
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17337204-Animals,
pubmed-meshheading:17337204-Aquaporins,
pubmed-meshheading:17337204-Blotting, Western,
pubmed-meshheading:17337204-Cell Hypoxia,
pubmed-meshheading:17337204-Cell Line,
pubmed-meshheading:17337204-Cell Size,
pubmed-meshheading:17337204-Hypotonic Solutions,
pubmed-meshheading:17337204-Immunohistochemistry,
pubmed-meshheading:17337204-Osmotic Pressure,
pubmed-meshheading:17337204-Phloretin,
pubmed-meshheading:17337204-RNA, Messenger,
pubmed-meshheading:17337204-Rats,
pubmed-meshheading:17337204-Retinal Ganglion Cells,
pubmed-meshheading:17337204-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17337204-Stress, Physiological,
pubmed-meshheading:17337204-Time Factors,
pubmed-meshheading:17337204-Up-Regulation,
pubmed-meshheading:17337204-Water-Electrolyte Balance
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pubmed:year |
2007
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pubmed:articleTitle |
Stress-induced changes in neuronal Aquaporin-9 (AQP9) in a retinal ganglion cell-line.
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pubmed:affiliation |
Department of Pharmacology & Neuroscience, University of North Texas Health Science Center at Fort Worth, TX 76107, USA. adibas@hsc.unt.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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