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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2007-4-3
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pubmed:abstractText |
Polymerase chain reaction (PCR) using DNA from blood samples is a valuable tool in the field of medical diagnostics. However, DNA isolation from blood is a laborious and sample-consuming step, and hampers the automation of PCR for large-scale studies. Attempts to perform PCR from blood without DNA isolation have been difficult to achieve, since numerous endogenous and exogenous blood constituents may inhibit PCR.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0009-8981
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
380
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
112-7
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pubmed:meshHeading |
pubmed-meshheading:17331487-Buffers,
pubmed-meshheading:17331487-DNA,
pubmed-meshheading:17331487-DNA Primers,
pubmed-meshheading:17331487-HIV-1,
pubmed-meshheading:17331487-Humans,
pubmed-meshheading:17331487-Indicators and Reagents,
pubmed-meshheading:17331487-Polymerase Chain Reaction,
pubmed-meshheading:17331487-Reproducibility of Results,
pubmed-meshheading:17331487-Sensitivity and Specificity,
pubmed-meshheading:17331487-Taq Polymerase,
pubmed-meshheading:17331487-Tumor Suppressor Protein p53
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pubmed:year |
2007
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pubmed:articleTitle |
A novel buffer system, AnyDirect, can improve polymerase chain reaction from whole blood without DNA isolation.
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pubmed:affiliation |
Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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