Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-3-2
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF080096, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF352359, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF352360, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF352361, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF352362, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490256, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490257, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490258, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490261, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490262, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490263, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490264, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490265, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490266, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490267, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490268, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490269, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490270, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ490271
pubmed:abstractText
Amyloodiniosis, caused by the dinoflagellate ectoparasite Amyloodinium ocellatum, is one of the most serious diseases affecting marine fish in warm and temperate waters. Current diagnostic methods rely entirely on the microscopic identification of parasites on the skin or gills of infested fish. However, subclinical infestations usually go undetected, while no method of detecting the free-swimming, infective (dinospore) stage has been devised. Targeting the parasite's ribosomal DNA region, we have developed a sensitive and specific PCR assay that can detect as little as a single cell from any of the 3 stages of the parasite's life cycle (trophont, tomont, dinospore). This assay performs equally well in a simple artificial seawater medium and in natural seawater containing a plankton community assemblage. The assay is also not inhibited by gill tissue. Sequence analysis of the internal transcribed spacer region of 5 A. ocellatum isolates, obtained from fish in the Red Sea (Israel), eastern Mediterranean Sea (Israel), Adriatic Sea (Italy), Gulf of Mexico (Florida), and from an unknown origin, revealed insignificant variation, indicating that all isolates were the same species. However, 3 of these isolates propagated in cell culture varied in behavior and morphology, and these differences were consistent during at least 2 yr in culture. Thus, our findings do not eliminate the possibility that different strains are in fact 'subspecies' or lower taxa, which may also differ in pathogenic and immunogenic characteristics, environmental tolerance, and other features.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0177-5103
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
219-26
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
A highly specific PCR assay for detecting the fish ectoparasite Amyloodinium ocellatum.
pubmed:affiliation
College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, North Carolina 27606-1499, USA. mike_levy@ncsu.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't