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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1992-2-18
|
| pubmed:abstractText |
Nuclear factor kappa-B (NF-kappa B) has been shown to play an important role in LPS-mediated induction of several genes in macrophages. Several studies have implicated protein kinase C (PKC) or cAMP-dependent protein kinase in the regulation of NF-kappa B activity. In this study we have investigated the mechanism of NF-kappa B induction in murine macrophages. A chloramphenicol acetyl transferase (CAT) expression vector containing multiple copies of the TNF-alpha NF-kappa B element was transfected into the RAW264 macrophage-like cell line and assessed for inducible CAT activity. LPS treatment of the transfected cells resulted in a significant induction of CAT activity. CAT activity was not induced by treatment with phorbol myristate acetate (PMA) or the cAMP analogue 8-bromo cAMP. To further study NF-kappa B induction, nuclear extracts were prepared from RAW264 cells. Extracts from RAW264 cells that were treated from 30 min to 2 hr with LPS had a significant increase in NF-kappa B binding activity as determined by the electrophoresis mobility shift assay (EMSA). Treatment of these cells from 30 min to 2 hr with PMA did not result in such binding activity. U.V. crosslinking analysis of the DNA-binding activity confirmed these results and indicated that LPS induced a 55 KD DNA-binding protein. Induction of this NF-kappa B binding activity was not inhibited by pretreatment with the PKC inhibitor H-7. H-7 did inhibit induction of TPA responsive element binding by either LPS or PMA. Prolonged exposure to phorbol ester, a treatment which down-regulates PKC, had no effect on LPS induction of NF-kappa B activity in these cells. These results suggest that the induction of NF-kappa B in macrophages by LPS is independent of PKC.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(5-Isoquinolinesulfonyl)-2-Methylp...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0021-9541
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
150
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
204-13
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1730783-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine,
pubmed-meshheading:1730783-Animals,
pubmed-meshheading:1730783-Base Sequence,
pubmed-meshheading:1730783-Cell Line,
pubmed-meshheading:1730783-DNA,
pubmed-meshheading:1730783-Down-Regulation,
pubmed-meshheading:1730783-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1730783-Isoquinolines,
pubmed-meshheading:1730783-Kinetics,
pubmed-meshheading:1730783-Lipopolysaccharides,
pubmed-meshheading:1730783-Macrophages,
pubmed-meshheading:1730783-Mice,
pubmed-meshheading:1730783-Molecular Sequence Data,
pubmed-meshheading:1730783-Molecular Weight,
pubmed-meshheading:1730783-NF-kappa B,
pubmed-meshheading:1730783-Piperazines,
pubmed-meshheading:1730783-Protein Kinase C,
pubmed-meshheading:1730783-Tetradecanoylphorbol Acetate
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Regulation of NF-kappa B activity in murine macrophages: effect of bacterial lipopolysaccharide and phorbol ester.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, S.U.N.Y. Health Science Center, Syracuse 13210.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|