Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-5-8
pubmed:abstractText
The unique IR3 gene of equine herpesvirus 1 (EHV-1) is expressed as a late 1.0-kb transcript. Previous studies confirmed the IR3 transcription initiation site and tentatively identified other cis-acting elements specific to IR3 such as a TATA box, a 443 base pair 5'untranslated region (UTR), a 285 base pair open reading frame (ORF), and a poly adenylation (A) signal [Holden, V.R., Harty, R.N., Yalamanchili, R.R., O'Callaghan, D.J., 1992. The IR3 gene of equine herpesvirus type 1: a unique gene regulated by sequences within the intron of the immediate-early gene. DNA Seq. 3, 143-152]. Transient transfection assays revealed that the IR3 promoter is strongly trans-activated by the IE protein (IEP) and that coexpression of the IEP with the early EICP0 and IR4 regulatory proteins results in maximal trans-activation of the IR3 promoter. Gel shift assays revealed that the IEP directly binds to the IR3 promoter region. Western blot analysis showed that the IR3 protein produced in E. coli was detected by antibodies to IR3 synthetic peptides; however, the IR3 protein was not detected in EHV-1 infected cell extracts by these same anti-IR3 antibodies, even though the IR3 transcript was detected by northern blot. These findings suggest that the IR3 may not be expressed to a protein. Expression of an IR3/GFP fusion gene was not observed, but expression of a GFP/IR3 fusion gene was detected by fluorescent microscopy. In further attempts to detect the IR3/GFP fusion protein using anti-GFP antibody, western blot analysis showed that the IR3/GFP fusion protein was not detected in vivo. Interestingly, a truncated form of the GFP/IR3 protein was synthesized from the GFP/IR3 fusion gene. However, GFP/IR3 and IR3/GFP fusion proteins of the predicted sizes were synthesized by in vitro coupled transcription and translation of the fusion genes, suggesting poor expression of the IR3 protein in vivo. The possible role of the IR3 transcript in EHV-1 infection is discussed.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-01, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-019001, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-02, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-027943, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-029001, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-03, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-035784, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-035790, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-039001, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-04, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-047369, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-047370, http://linkedlifedata.com/resource/pubmed/grant/P20 RR018724-05, http://linkedlifedata.com/resource/pubmed/grant/R01 AI022001-21, http://linkedlifedata.com/resource/pubmed/grant/R01 AI022001-22
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-10627530, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-10627553, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-11145900, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-11448176, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-11581390, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-11900834, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-12552007, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-1309921, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-1318606, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-1323700, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-1335300, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-14644612, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-14659563, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-15207618, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-15479811, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-15708594, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-1645793, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-16501071, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-16641295, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-16732484, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-2121064, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-2549711, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-2825444, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-2833023, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-2990102, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-3033896, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-3313277, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-6270904, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-6275028, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-6283147, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-7483272, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-7483278, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-7561779, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-7618267, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-7707500, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-7707529, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-8030239, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-8035487, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-8207808, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-8263911, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-8380457, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-8995619, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-9143293, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-9188552, http://linkedlifedata.com/resource/pubmed/commentcorrection/17306852-9705258
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
363
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-25
pubmed:dateRevised
2009-12-31
pubmed:meshHeading
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