Linked Life Data

1730390

Source:http://linkedlifedata.com/resource/pubmed/id/1730390

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-2-20
pubmed:databankReference
pubmed:abstractText
Sequence analysis of cDNA clones encoding fibronectin (FN) from Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternative spliced V region of the protein. These cDNAs have been used to study the expression of FN mRNAs during early development. Overall, levels of maternal FN mRNA remain constant until the mid- to late-gastrula stage when the accumulation of new FN transcripts is first apparent. RNase protection analyses reveal that the pattern of FN alternative splicing is similar to that reported for other species and does not change with the shift from maternal to zygotic mRNA expression. The cellular forms of the FN protein predominate in the early embryo with the EIIIA and EIIIB exons included in most mRNAs at this time. A comparison of V-region alternative splicing between embryonic and adult liver RNAs indicates a segment of 345 nucleotides that can be either completely excluded or included in mature FN transcripts but there is no evidence for additional V-region variants. Maternal mRNAs encoding alternatively spliced forms of FN can be specifically eliminated from Xenopus oocytes following the injection of antisense oligodeoxynucleotides into the cytoplasm, thereby making it possible to analyze the structure, composition, and function of FN mRNAs in early embryos.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
357-69
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Identification and characterization of alternatively spliced fibronectin mRNAs expressed in early Xenopus embryos.
pubmed:affiliation
Department of Anatomy and Cell Biology, University of Virginia Health Sciences Center, Charlottesville 22908.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't