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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-4-25
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pubmed:abstractText |
A nonsynonymous single nucleotide polymorphism (SNP) of the low-affinity IgE receptor (FcvarepsilonRII/CD23) gene resulting in an arginine to tryptophan exchange at amino-acid position 62 (R62W) has been associated with enhanced T-cell responses to antigen in allergic subjects. To explore the mechanism, a CD23(a) cDNA was cloned into the plasmid pCMVScript-CD23a-C with a C allele (R62). The pCMVScript-CD23a-T with T (W62) was produced using a site-directed mutagenesis approach. The pCMVScript-CD23a-C only (CC), mixture of pCMVScript-CD23a-T and pCMVSCript-CD23a-C (CT) and pCMVScript-CD23a-T only (TT) plasmids were transfected in Cos-7 cells at equivalence in transfection efficiency. No soluble CD23 was released from TT transfectants whereas a higher level of soluble CD23 was detected in CC than in CT transfectants. Human leukocyte elastase (HLE), cathepsin G, the dust mite allergen Der p I and ADAM 33 (A disintegrin and metalloproteinase) were found to cleave membrane CD23 in CC but not in TT transfectants, implying the resistance of CD23 to enzymatic cleavage associated with T mutant. Addition of tunicamycin resulted in the resistance of CD23 to Der p I mediated cleavage in CC but no change in TT transfectants. These results indicate that R62W influences the stability of membrane CD23 molecules due to possibly diminished N-glycosylation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADAM Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ADAM33 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Dermatophagoides,
http://linkedlifedata.com/resource/pubmed/chemical/Arthropod Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CTSG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin G,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsins,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Dermatophagoides pteronyssinus...,
http://linkedlifedata.com/resource/pubmed/chemical/Leukocyte Elastase,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Tunicamycin
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1466-4879
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-23
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:17301828-ADAM Proteins,
pubmed-meshheading:17301828-Amino Acid Sequence,
pubmed-meshheading:17301828-Amino Acid Substitution,
pubmed-meshheading:17301828-Animals,
pubmed-meshheading:17301828-Antigens, Dermatophagoides,
pubmed-meshheading:17301828-Arthropod Proteins,
pubmed-meshheading:17301828-Base Sequence,
pubmed-meshheading:17301828-COS Cells,
pubmed-meshheading:17301828-Cathepsin G,
pubmed-meshheading:17301828-Cathepsins,
pubmed-meshheading:17301828-Cercopithecus aethiops,
pubmed-meshheading:17301828-Cysteine Endopeptidases,
pubmed-meshheading:17301828-DNA, Complementary,
pubmed-meshheading:17301828-Glycosylation,
pubmed-meshheading:17301828-Humans,
pubmed-meshheading:17301828-Leukocyte Elastase,
pubmed-meshheading:17301828-Polymorphism, Single Nucleotide,
pubmed-meshheading:17301828-Receptors, IgE,
pubmed-meshheading:17301828-Recombinant Proteins,
pubmed-meshheading:17301828-Serine Endopeptidases,
pubmed-meshheading:17301828-Transfection,
pubmed-meshheading:17301828-Tunicamycin
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pubmed:year |
2007
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pubmed:articleTitle |
Polymorphism R62W results in resistance of CD23 to enzymatic cleavage in cultured cells.
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pubmed:affiliation |
1Pediatric Immunology Research Department, Children's Mercy Hospital/School of Medicine, University of Missouri at Kansas City, Kansas City, MO, USA. jmeng@cmh.edu
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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