Linked Life Data

17296219

Source:http://linkedlifedata.com/resource/pubmed/id/17296219

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2007-2-27
pubmed:abstractText
Although synthetic biomaterials have a wide range of promising applications in regenerative medicine and tissue engineering, there is limited insight into the basic materials properties that influence cellularisation events. The aim of this study was to investigate the influence of the physical properties of polyester films on the adherence and growth of normal human urothelial and urinary smooth muscle (SM) cells, as part of a programme for the development of potential biomaterials for bladder tissue engineering. Films of different thickness were produced by spin coating from solution. Cell attachment and proliferation were analysed and revealed a reproducible and significant growth advantage over the initial 7 days for both cell types on poly(lactide-co-glycolide) (PLGA) versus poly(epsilon-caprolactone) (PCL), and on thick versus thin films. In order to understand the basis of the variation in cell growth, the surface morphology, degradation behaviour and mechanical properties of the films were investigated. The pattern of cell attachment and growth was found to be unrelated to surface topography and no distinction in film degradation behaviour was found to account for differences in cell growth, except at late time points (14 days), where degradation of thin PLGA films became significant. By contrast, the flexural loss and storage moduli were found to be reduced in films composed of PLGA versus PCL, and also as film thickness increased, indicating that mechanical properties of biomaterials can influence cell growth. We conclude that elastic modulus is relevant to biology at the cellular scale and may also be influential at the tissue/organ level, and is a critical parameter to be considered during development of synthetic biomaterials for tissue engineering.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2264-74
pubmed:meshHeading
pubmed-meshheading:17296219-Biocompatible Materials, pubmed-meshheading:17296219-Biomechanics, pubmed-meshheading:17296219-Calorimetry, Differential Scanning, pubmed-meshheading:17296219-Cell Adhesion, pubmed-meshheading:17296219-Cell Count, pubmed-meshheading:17296219-Cell Culture Techniques, pubmed-meshheading:17296219-Cell Proliferation, pubmed-meshheading:17296219-Cells, Cultured, pubmed-meshheading:17296219-Fluorescent Antibody Technique, pubmed-meshheading:17296219-Hexanoic Acids, pubmed-meshheading:17296219-Humans, pubmed-meshheading:17296219-Lactic Acid, pubmed-meshheading:17296219-Lactones, pubmed-meshheading:17296219-Molecular Weight, pubmed-meshheading:17296219-Muscle, Smooth, pubmed-meshheading:17296219-Polyesters, pubmed-meshheading:17296219-Polyglycolic Acid, pubmed-meshheading:17296219-Polymers, pubmed-meshheading:17296219-Spectroscopy, Fourier Transform Infrared, pubmed-meshheading:17296219-Time Factors, pubmed-meshheading:17296219-Tissue Engineering, pubmed-meshheading:17296219-Urogenital System
pubmed:year
2007
pubmed:articleTitle
Influence of the physical properties of two-dimensional polyester substrates on the growth of normal human urothelial and urinary smooth muscle cells in vitro.
pubmed:affiliation
IRC in Polymer Science and Technology, Department of Chemistry, Durham University, South Road, Durham DH1 3LE, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't