rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
|
pubmed:dateCreated |
1992-2-13
|
pubmed:abstractText |
Transcriptionally active regions of the Haloferax volcanii genome were mapped by hybridization of radiolabeled cDNA to Southern blots of our minimal set of overlapping cosmid clones covering 96% of the 4.1-Mbp genome. Transcription during exponential growth occurred in nearly every region of the 2,920-kbp chromosome. Large parts of the 690- and the 86-kbp plasmids were transcribed, but the 440-kbp plasmid showed little expression. Transcription after a 40-min heat shock at 65 degrees C was generally reduced, apart from a small set of strongly expressed loci all situated on the chromosome.
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pubmed:commentsCorrections |
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0021-9193
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
174
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
30-4
|
pubmed:dateRevised |
2010-9-9
|
pubmed:meshHeading |
pubmed-meshheading:1729217-Archaea,
pubmed-meshheading:1729217-Bacterial Proteins,
pubmed-meshheading:1729217-Blotting, Southern,
pubmed-meshheading:1729217-Cell Division,
pubmed-meshheading:1729217-Chromosome Mapping,
pubmed-meshheading:1729217-Cloning, Molecular,
pubmed-meshheading:1729217-Cosmids,
pubmed-meshheading:1729217-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:1729217-Genome, Bacterial,
pubmed-meshheading:1729217-Heat-Shock Proteins,
pubmed-meshheading:1729217-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Transcriptionally active regions in the genome of the archaebacterium Haloferax volcanii.
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pubmed:affiliation |
Department of Biology, University of Ottawa, Ontario, Canada.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|