Linked Life Data

17262819

Source:http://linkedlifedata.com/resource/pubmed/id/17262819

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-4-17
pubmed:abstractText
In osteoarthritis (OA), hepatocyte growth factor (HGF) is supposed to play a role in cartilage repair. Because the development of osteophytes is a major characteristic of OA and thought to be part of an attempted repair process, the purpose of this study was to determine whether HGF may be involved in osteophyte formation. HGF levels in synovial fluids from 41 patients assessed by enzyme immunosorbant assay were correlated with disease severity and osteophyte formation, evaluated by anteroposterior weight-bearing radiographs. Detection of HGF, c-Met, and CD68 in cartilage and synovial tissues was assessed by immunohistochemistry. Effects of HGF on the secretion of TGF-beta1 and BMP-2 by chondrocytes, fibroblast-like synovial cells (FLS), and macrophages as well as HGF-induced secretion of MCP-1 by FLS and chondrocytes were determined by ELISA. HGF was detected in all synovial fluids and concentrations correlated highly with disease severity and osteophyte formation (p < 0.001). Immunohistochemistry revealed weak synovial staining for HGF, whereas increasing numbers of HGF expressing chondrocytes were detected depending on disease severity. In addition, an increased number of macrophages in synovial specimens was observed, which was likewise severity dependent. In a series of subsequent in vitro studies, HGF remarkable induced MCP-1 secretion by FLS in a dose-dependent manner. No effect on TGF-beta1 and BMP-2 secretion by FLS and chondrocytes was evident upon HGF stimulation, whereas secretion of these growth factors by PMA-differentiated THP-1 cells was significantly increased by HGF. The results indicate that HGF may facilitate osteophyte development by promoting MCP-1-mediated entry of monocytes/macrophages into the OA-affected joint and/or by stimulating macrophage-derived growth factors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0736-0266
pubmed:author
pubmed:copyrightInfo
(c) 2007 Orthopaedic Research Society.
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
569-77
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17262819-Adult, pubmed-meshheading:17262819-Aged, pubmed-meshheading:17262819-Aged, 80 and over, pubmed-meshheading:17262819-Antigens, CD, pubmed-meshheading:17262819-Antigens, Differentiation, Myelomonocytic, pubmed-meshheading:17262819-Cartilage, pubmed-meshheading:17262819-Cells, Cultured, pubmed-meshheading:17262819-Chemokine CCL2, pubmed-meshheading:17262819-Chondrocytes, pubmed-meshheading:17262819-Female, pubmed-meshheading:17262819-Femur, pubmed-meshheading:17262819-Hepatocyte Growth Factor, pubmed-meshheading:17262819-Humans, pubmed-meshheading:17262819-Macrophages, pubmed-meshheading:17262819-Male, pubmed-meshheading:17262819-Middle Aged, pubmed-meshheading:17262819-Monocytes, pubmed-meshheading:17262819-Osteoarthritis, pubmed-meshheading:17262819-Proto-Oncogene Proteins c-met, pubmed-meshheading:17262819-Severity of Illness Index, pubmed-meshheading:17262819-Synovial Fluid, pubmed-meshheading:17262819-Synovial Membrane
pubmed:year
2007
pubmed:articleTitle
Hepatocyte growth factor induction of macrophage chemoattractant protein-1 and osteophyte-inducing factors in osteoarthritis.
pubmed:affiliation
Department of Orthopaedics, University Hospital of Muenster, Domagkstrasse 3, D-48149 Muenster, Germany. dankarb@uni-muenster.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't