|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0017428,
umls-concept:C0028606,
umls-concept:C0075804,
umls-concept:C0185125,
umls-concept:C0220781,
umls-concept:C0301869,
umls-concept:C0392762,
umls-concept:C0577559,
umls-concept:C0936012,
umls-concept:C1883254,
umls-concept:C2349943
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2007-3-29
|
|
pubmed:abstractText |
The synthesis and characterization of isotopomer tandem nucleic acid mass tag-peptide nucleic acid (TNT-PNA) conjugates is described along with their use as electrospray ionisation-cleavable (ESI-Cleavable) hybridization probes for the detection and quantification of target DNA sequences by electrospray ionisation tandem mass spectrometry (ESI-MS/MS). ESI-cleavable peptide TNT isotopomers were introduced into PNA oligonucleotide sequences in a total synthesis approach. These conjugates were evaluated as hybridization probes for the detection and quantification of immobilized synthetic target DNAs using ESI-MS/MS. In these experiments, the PNA portion of the conjugate acts as a hybridization probe, whereas the peptide TNT is released in a collision-based process during the ionization of the probe conjugate in the electrospray ion source. The cleaved TNT acts as a uniquely resolvable marker to identify and quantify a unique target DNA sequence. The method should be applicable to a wide variety of assays requiring highly multiplexed, quantitative DNA/RNA analysis, including gene expression monitoring, genetic profiling and the detection of pathogens.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-10047504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-10504701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-10556319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-10780402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-11172497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-11180628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-11180630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-11840562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-12510732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-12510733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-12694726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-12713048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-1451687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-15307768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-15329876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-15757550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-15774555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-15859613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-15911764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-15997299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-16100268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-16448044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-16503155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-16670426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-7667088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-8199231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-8512946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-8689239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9115363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9278502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9286165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9322432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9337595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9358169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9360490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9415888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9555731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17259215-9631064
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1362-4962
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
35
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
e28
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Electrospray ionisation-cleavable tandem nucleic acid mass tag-peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS.
|
|
pubmed:affiliation |
Trillion Genomics Ltd, Babraham Research Campus, Babraham, Cambridge CB2 4AT, UK.
|
|
pubmed:publicationType |
Journal Article,
Evaluation Studies
|
