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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1976-3-1
|
| pubmed:abstractText |
Calcium concentrations of various pancreatic B cell organelles have been determined by X-ray microanalysis of areas of frozen sections of unfixed rat islets of Langerhans. Highest concentrations were detected in storage granules and in mitochondria, although calcium was also present in nuclei, in areas of endoplasmic reticulum and of cytoplasm. Accumulation of 45Ca by isolated organelles has been studied in homogenates and isolated subcellular fractions of rat islets of Langerhans. In the presence of a permeant anion (oxalate or phosphate), accumulation of 45Ca into mitochondria and microsomes was strongly stimulated by ATP. This net uptake was diminished during incubation of homogenates or of a mitochondria plus storage granule-rich fraction in the presence of cyclic AMP, dibutyryl cyclic GMP; 2:4-dinitrophenol or of ruthenium red. Investigations of the characteristics of 45Ca accumulation by homogenates prepared from storage granule-depleted islets showed no differences from those of normal islets, suggesting that the granules do not represent an important labile pool of calcium. With the exception of cyclic AMP and cyclic GMP none of the insulin secretagogues tested (glucose, leucine, arginine, adrenalin, noradrenalin, theophylline, glibenclamide) altered calcium accumulation by islet homogenates. On the basis of absolute calcium levels and of 45Ca uptake studies it is concluded that islet B cells contain a readily exchangeable mitochondrial calcium pool, and an endoplasmic reticulum pool containing a lower concentration of calcium which is also readily exchangeable. The storage granules, despite their high calcium content, do not appear to constitute a labile pool. It seems likely that the labile mitochondria and endoplasmic reticulum pools play a predominant role in the regulation of cytoplasmic free calcium levels, which may in turn be important in the regulation of rates of insulin secretion.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Monophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Caffeine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP,
http://linkedlifedata.com/resource/pubmed/chemical/Theophylline,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthines
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0021-9533
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
395-409
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:172523-Adenosine Monophosphate,
pubmed-meshheading:172523-Adenosine Triphosphate,
pubmed-meshheading:172523-Animals,
pubmed-meshheading:172523-Biological Transport, Active,
pubmed-meshheading:172523-Bucladesine,
pubmed-meshheading:172523-Caffeine,
pubmed-meshheading:172523-Calcium,
pubmed-meshheading:172523-Cell Nucleus,
pubmed-meshheading:172523-Cyclic AMP,
pubmed-meshheading:172523-Cyclic GMP,
pubmed-meshheading:172523-Cytoplasm,
pubmed-meshheading:172523-Cytoplasmic Granules,
pubmed-meshheading:172523-Endoplasmic Reticulum,
pubmed-meshheading:172523-Female,
pubmed-meshheading:172523-Islets of Langerhans,
pubmed-meshheading:172523-Mitochondria,
pubmed-meshheading:172523-Rats,
pubmed-meshheading:172523-Subcellular Fractions,
pubmed-meshheading:172523-Theophylline,
pubmed-meshheading:172523-Xanthines
|
| pubmed:year |
1975
|
| pubmed:articleTitle |
Calcium distribution in islets of Langerhans: a study of calcium concentrations and of calcium accumulation in B cell organelles.
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| pubmed:publicationType |
Journal Article
|