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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-4-13
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pubmed:abstractText |
The paper describes a sensitive latex hybridization assay (LHA) method applied for indirect detection of biotinylated nucleic acid hybrids immobilized on a synthetic membrane. The biotinylated hybrids were visualized by means of latex particles containing the fluorescent dye pyronine G and coated with streptavidin; 1.6 and 0.3 pg of lambda-phage DNA was detected by dot blot hybridizations on nylon membrane and polyethyleneimine-cellophane, respectively. The assay sensitivity was increased by three orders of magnitude over that with fluorescently labeled probes due to encapsulation of the fluorescent dye in polymer particles. LHA is simple (single-stage detection procedure), fast, and more sensitive than any of the other nonradioactive hybridization methods.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acrolein,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Biotin,
http://linkedlifedata.com/resource/pubmed/chemical/Cellophane,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Latex,
http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial,
http://linkedlifedata.com/resource/pubmed/chemical/Nylons,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethyleneimine,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Streptavidin,
http://linkedlifedata.com/resource/pubmed/chemical/polyacrolein
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
198
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
308-11
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pubmed:dateRevised |
2006-4-17
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pubmed:meshHeading |
pubmed-meshheading:1724721-Acrolein,
pubmed-meshheading:1724721-Bacterial Proteins,
pubmed-meshheading:1724721-Biological Markers,
pubmed-meshheading:1724721-Biotin,
pubmed-meshheading:1724721-Cellophane,
pubmed-meshheading:1724721-DNA,
pubmed-meshheading:1724721-Fluorescent Dyes,
pubmed-meshheading:1724721-Latex,
pubmed-meshheading:1724721-Membranes, Artificial,
pubmed-meshheading:1724721-Nucleic Acid Hybridization,
pubmed-meshheading:1724721-Nylons,
pubmed-meshheading:1724721-Polyethyleneimine,
pubmed-meshheading:1724721-Polymers,
pubmed-meshheading:1724721-Staining and Labeling,
pubmed-meshheading:1724721-Streptavidin
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pubmed:year |
1991
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pubmed:articleTitle |
A novel approach to nonradioactive hybridization assay of nucleic acids using stained latex particles.
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pubmed:affiliation |
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
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pubmed:publicationType |
Journal Article
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