Linked Life Data

17237528

Source:http://linkedlifedata.com/resource/pubmed/id/17237528

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-1-22
pubmed:abstractText
Our understanding of basic cell structure and function has been greatly aided by the identification of proteins at the ultrastructural level. However, the current methods for high-resolution labeling of proteins in situ, and for directly correlating observations made by light microscopy (LM) and electron microscopy (EM) although invaluable, have a number of substantial limitations. These range from poor label penetration, difficulty to perform simultaneous multiprotein labeling, or the need to take the samples all the way to the electron microscope to evaluate labeling efficacy. Here we demonstrate an approach using quantum dots for pre-embedding immunolabeling of multiple diverse proteins for both LM and EM that overcomes many of these problems.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
374
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-53
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Light and electron microscopic localization of multiple proteins using quantum dots.
pubmed:affiliation
Department of Neurosciences, The National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, California, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural