Linked Life Data

1723677

Source:http://linkedlifedata.com/resource/pubmed/id/1723677

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-3-13
pubmed:abstractText
In this paper, we report on the preparation and application of anti-human heterogeneous AFP-R-LCA monoclonal antibodies (VG5, VD12, VB5, VA8, VD1). These McAbs were more sensitive and specific for AFP-R-LCA than the anti-AFP polyclonal antibody routinely used. The twin site (a and b) sandwich ELISA method so established was used to test the serum samples of 69 PHC patients, 67 patients with benign liver diseases, 30 pregnant women and 30 normal controls. The results showed that this twin site sandwich ELISA method gave a false positive reaction in only 2.1% and was 81.2% (56/69) positive reaction, giving a positive reaction to 5-100 ng/ml. It was positive in PHC patients with AFP levels less than 400 ng/ml. This method, being simple, accurate and reliable, is valuable in the differential diagnosis of PHC from benign liver diseases.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0253-3766
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
328-31
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
[Value of anti-human heterogeneous lens culinaris agglutinin reactive AFP monoclonal antibody in the diagnosis of primary hepatocellular carcinoma (PHC)].
pubmed:affiliation
Institute of Hepatobiliary Surgery, Second Military Medical University, Shanghai.
pubmed:publicationType
Journal Article, English Abstract