| pubmed-article:17229887 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C0027215 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C0007608 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C0071253 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C1166758 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C1167214 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C1420236 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C1704666 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C1517892 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C0297119 | lld:lifeskim |
| pubmed-article:17229887 | lifeskim:mentions | umls-concept:C0208973 | lld:lifeskim |
| pubmed-article:17229887 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:17229887 | pubmed:dateCreated | 2007-3-29 | lld:pubmed |
| pubmed-article:17229887 | pubmed:abstractText | Using phage display, we identified Na+/H+ exchanger regulatory factor (NHERF)-2 as a novel binding partner for the cadherin-associated protein, beta-catenin. We showed that the second of two PSD-95/Dlg/ZO-1 (PDZ) domains of NHERF interacts with a PDZ-binding motif at the very carboxy terminus of beta-catenin. N-cadherin expression has been shown to induce motility in a number of cell types. The first PDZ domain of NHERF is known to bind platelet-derived growth factor-receptor beta (PDGF-Rbeta), and the interaction of PDGF-Rbeta with NHERF leads to enhanced cell spreading and motility. Here we show that beta-catenin and N-cadherin are in a complex with NHERF and PDGF-Rbeta at membrane ruffles in the highly invasive fibrosarcoma cell line HT1080. Using a stable short hairpin RNA system, we showed that HT1080 cells knocked down for either N-cadherin or NHERF had impaired ability to migrate into the wounded area in a scratch assay, similar to cells treated with a PDGF-R kinase inhibitor. Cells expressing a mutant NHERF that is unable to associate with beta-catenin had increased stress fibers, reduced lamellipodia, and impaired cell migration. Using HeLa cells, which express little to no PDGF-R, we introduced PDGF-Rbeta and showed that it coimmunoprecipitates with N-cadherin and that PDGF-dependent cell migration was reduced in these cells when we knocked-down expression of N-cadherin or NHERF. These studies implicate N-cadherin and beta-catenin in cell migration via PDGF-R-mediated signaling through the scaffolding molecule NHERF. | lld:pubmed |
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| pubmed-article:17229887 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17229887 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17229887 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17229887 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17229887 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:17229887 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17229887 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:17229887 | pubmed:issn | 1059-1524 | lld:pubmed |
| pubmed-article:17229887 | pubmed:author | pubmed-author:WahlJames... | lld:pubmed |
| pubmed-article:17229887 | pubmed:author | pubmed-author:JohnsonKeith... | lld:pubmed |
| pubmed-article:17229887 | pubmed:author | pubmed-author:WheelockMarga... | lld:pubmed |
| pubmed-article:17229887 | pubmed:author | pubmed-author:TheisenChrist... | lld:pubmed |
| pubmed-article:17229887 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17229887 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:17229887 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17229887 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17229887 | pubmed:pagination | 1220-32 | lld:pubmed |
| pubmed-article:17229887 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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