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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1992-1-9
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pubmed:abstractText |
The 20-amino acid peptide M-15 binds with high affinity (IC50 approximately 0.1 nM) to 125I-labeled galanin (125I-GAL) binding sites in membranes from the ventral hippocampus, midbrain, and rat spinal cord. Receptor autoradiographic studies show that M-15 can displace 125I-GAL from all labeled sites. M-15 acts as a reversible high-affinity antagonist in blocking the inhibitory effects of GAL on the evoked release of acetylcholine in vivo in the hippocampus and on the GAL-induced hyperpolarization of locus coeruleus neurons in slices. M-15 also blocks the facilitatory effects of GAL on the spinal flexor reflex. Thus, the chimeric peptide M-15 [GAL-(1-13)-substance P-(5-11)amide] represents the first antagonist to the neuronal actions of GAL.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-228806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2416401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2426125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2432203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2436195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2444980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2455255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2462255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2471574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2474449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2474450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2477035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2479930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-2594786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-3719339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-6197320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-6493483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-7430440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-7430441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1720557-922502
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
10961-5
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:1720557-Acetylcholine,
pubmed-meshheading:1720557-Animals,
pubmed-meshheading:1720557-Autoradiography,
pubmed-meshheading:1720557-Binding, Competitive,
pubmed-meshheading:1720557-Binding Sites,
pubmed-meshheading:1720557-Cell Membrane,
pubmed-meshheading:1720557-Chimera,
pubmed-meshheading:1720557-Electric Conductivity,
pubmed-meshheading:1720557-Galanin,
pubmed-meshheading:1720557-Hippocampus,
pubmed-meshheading:1720557-Iodine Radioisotopes,
pubmed-meshheading:1720557-Kinetics,
pubmed-meshheading:1720557-Locus Coeruleus,
pubmed-meshheading:1720557-Male,
pubmed-meshheading:1720557-Membrane Potentials,
pubmed-meshheading:1720557-Neurons,
pubmed-meshheading:1720557-Neuropeptides,
pubmed-meshheading:1720557-Peptides,
pubmed-meshheading:1720557-Rats,
pubmed-meshheading:1720557-Rats, Inbred Strains,
pubmed-meshheading:1720557-Receptors, Galanin,
pubmed-meshheading:1720557-Receptors, Gastrointestinal Hormone,
pubmed-meshheading:1720557-Spinal Cord,
pubmed-meshheading:1720557-Substance P
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pubmed:year |
1991
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pubmed:articleTitle |
M-15: high-affinity chimeric peptide that blocks the neuronal actions of galanin in the hippocampus, locus coeruleus, and spinal cord.
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pubmed:affiliation |
Department of Biochemistry, Arrheniuslaboratories, Stockholm University, Sweden.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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