Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2006-12-15
pubmed:abstractText
Terminally differentiated adult injured cardiac myocytes have been used for various animal models of heart failure. It has recently been shown that isoproterenol induces injury in rat neonatal cardiac myocytes via a beta-adrenergic pathway, suggesting that it might be one of the factors involved in myocardial cell injury in heart failure in vivo. In the study, silibinin, a plant flavanoid from milk thistle was first evaluated for its protective effect against beta-adrenergic agonist isoproterenol-induced injury in cultured rat neonatal cardiac myocytes. The viability, activation of lactate dehydrogenase (LDH), and content of maleic dialdehyde (MDA) were chosen for measuring the degree of cardiac myocytes injury. As a result, silibinin protected isoproterenol-treated rat cardiac myocytes from death and significantly decreased LDH release and MDA production. Silibinin increased superoxide dismutase activity, decreased [Ca(2+)](i), and increased mitochondrial membrane potential (DeltaPsi). Furthermore, the release of pro-apoptotic cytochrome c from mitochondria was reduced by silibinin. Silibinin increased the expression of anti-apoptotic Bcl-2 family protein Bcl-2, and up-regulation of SIRT1 inhibited the translocation of Bax from cytoplasm to mitochondria, which caused mitochondrial dysfunction and cell injury. These results demonstrate that silibinin protects against isoproterenol-induced cardiac myocytes injury through resuming mitochondrial function and regulating the expression of SIRT1 and Bcl-2 family members.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Agonists, http://linkedlifedata.com/resource/pubmed/chemical/Aldehydes, http://linkedlifedata.com/resource/pubmed/chemical/Bax protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Bcl2l1 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c, http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol, http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase, http://linkedlifedata.com/resource/pubmed/chemical/Protective Agents, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2, http://linkedlifedata.com/resource/pubmed/chemical/Silymarin, http://linkedlifedata.com/resource/pubmed/chemical/Sirt1 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Sirtuin 1, http://linkedlifedata.com/resource/pubmed/chemical/Sirtuins, http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase, http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein, http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein, http://linkedlifedata.com/resource/pubmed/chemical/malealdehyde, http://linkedlifedata.com/resource/pubmed/chemical/silybin
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1347-8613
pubmed:author
pubmed:issnType
Print
pubmed:volume
102
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
387-95
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17170512-Adrenergic beta-Agonists, pubmed-meshheading:17170512-Aldehydes, pubmed-meshheading:17170512-Animals, pubmed-meshheading:17170512-Animals, Newborn, pubmed-meshheading:17170512-Calcium, pubmed-meshheading:17170512-Cell Survival, pubmed-meshheading:17170512-Cells, Cultured, pubmed-meshheading:17170512-Cytochromes c, pubmed-meshheading:17170512-Dose-Response Relationship, Drug, pubmed-meshheading:17170512-Isoproterenol, pubmed-meshheading:17170512-L-Lactate Dehydrogenase, pubmed-meshheading:17170512-Membrane Potential, Mitochondrial, pubmed-meshheading:17170512-Mitochondria, Heart, pubmed-meshheading:17170512-Myocytes, Cardiac, pubmed-meshheading:17170512-Protective Agents, pubmed-meshheading:17170512-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:17170512-Rats, pubmed-meshheading:17170512-Rats, Sprague-Dawley, pubmed-meshheading:17170512-Silymarin, pubmed-meshheading:17170512-Sirtuin 1, pubmed-meshheading:17170512-Sirtuins, pubmed-meshheading:17170512-Superoxide Dismutase, pubmed-meshheading:17170512-Up-Regulation, pubmed-meshheading:17170512-bcl-2-Associated X Protein, pubmed-meshheading:17170512-bcl-X Protein
pubmed:year
2006
pubmed:articleTitle
Silibinin protects against isoproterenol-induced rat cardiac myocyte injury through mitochondrial pathway after up-regulation of SIRT1.
pubmed:affiliation
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang, China.
pubmed:publicationType
Journal Article