Linked Life Data

17158449

Source:http://linkedlifedata.com/resource/pubmed/id/17158449

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-2-5
pubmed:abstractText
Transforming growth factor beta-activated kinase 1 (TAK1), a member of the MAPKKK family, was initially described to play an essential role in the transforming growth factor beta-signaling pathway, but recent evidence has emerged implicating TAK1 in the interleukin (IL)-1 and tumor necrosis factor (TNF) pathways. Notably, two homologous proteins, TAB2 and TAB3, have been identified as adaptors linking TAK1 to the upstream adaptors TRAFs. However, it remains unclear whether the interaction between TAB2/TAB3 and TAK1 is necessary for its kinase activation and subsequent activation of the IKK and MAPK pathways. Here, we characterized the TAB2/TAB3-binding domain in TAK1 and further examined the requirement of this interaction for IL-1, TNF, and RANKL signaling. Through deletion mapping experiments, we demonstrated that the binding motif for TAB2/TAB3 is a non-contiguous region located within the last C-terminal 100 residues of TAK1. However, residues 479-553 of TAK1 appear to be necessary and sufficient for TAB2/TAB3 interaction. Conversely, residues 574-693 of TAB2 were shown to interact with TAK1. A green fluorescent protein fusion protein containing the last 100 residues of TAK1 (TAK1-C100) abolished the interaction of endogenous TAB2/TAB3 with TAK1, the phosphorylation of TAK1, and prevented the activation of IKK and MAPK induced by IL-1, TNF, and RANKL. Furthermore, TAK1-C100 blocked RANKL-induced nuclear accumulation of NFATc1 and consequently osteoclast differentiation consistent with the ability of a catalytically inactive TAK1 to block RANKL-mediated signaling. Significantly, our study provides evidence that the TAB2/TAB3 interaction with TAK1 is crucial for the activation of signaling cascades mediated by IL-1, TNF, and RANKL.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
282
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3918-28
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed-meshheading:17158449-Adaptor Proteins, Signal Transducing, pubmed-meshheading:17158449-Amino Acid Sequence, pubmed-meshheading:17158449-Animals, pubmed-meshheading:17158449-Cell Differentiation, pubmed-meshheading:17158449-Cell Line, pubmed-meshheading:17158449-Humans, pubmed-meshheading:17158449-Interleukin-1, pubmed-meshheading:17158449-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:17158449-L Cells (Cell Line), pubmed-meshheading:17158449-MAP Kinase Kinase Kinases, pubmed-meshheading:17158449-Mice, pubmed-meshheading:17158449-Molecular Sequence Data, pubmed-meshheading:17158449-Osteoclasts, pubmed-meshheading:17158449-Peptide Fragments, pubmed-meshheading:17158449-Protein Binding, pubmed-meshheading:17158449-Protein Structure, Tertiary, pubmed-meshheading:17158449-RANK Ligand, pubmed-meshheading:17158449-Sequence Deletion, pubmed-meshheading:17158449-Signal Transduction, pubmed-meshheading:17158449-Tumor Necrosis Factor-alpha
pubmed:year
2007
pubmed:articleTitle
TAK1-dependent signaling requires functional interaction with TAB2/TAB3.
pubmed:affiliation
Department of Experimental Therapeutics, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural