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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
30
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pubmed:dateCreated |
2006-12-20
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pubmed:abstractText |
The consequences of ultraviolet (UV) exposure are implicated in skin aging and cell death. The ribosomal protein S3 (rpS3) is one of the major proteins by which cells counteract the deleterious effects of UV and it plays a role in the repair of damaged DNA. In the present study, we investigated the protective effects of PEP-1-rpS3 fusion protein after UV-induced cell injury. A human rpS3 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-rpS3 fusion protein. The expressed and purified fusion proteins were efficiently transduced into skin cells in a time- and dose-dependent manner. Once inside the cells, transduced PEP-1-rpS3 fusion protein was stable for 48h. We showed that transduced PEP-1-rpS3 fusion protein increased cell viability and dramatically reduced DNA lesions in the UV exposed skin cells. Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin. These results suggest that PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-5793
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pubmed:author |
pubmed-author:AnJae JinJJ,
pubmed-author:ChoSung-WooSW,
pubmed-author:ChoiSoo HyunSH,
pubmed-author:ChoiSoo YoungSY,
pubmed-author:EumWon SikWS,
pubmed-author:JangSang HoSH,
pubmed-author:KangTae-CheonTC,
pubmed-author:KimDae WonDW,
pubmed-author:KimJoonJ,
pubmed-author:KimSo YoungSY,
pubmed-author:KwonHyung JooHJ,
pubmed-author:LeeKil SooKS,
pubmed-author:LeeNam IlNI,
pubmed-author:LeeSun HwaSH,
pubmed-author:ParkJinseuJ,
pubmed-author:RyuHea JinHJ,
pubmed-author:WonMoo HoMH,
pubmed-author:YeoSeung IlSI
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
580
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6755-62
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pubmed:dateRevised |
2010-6-16
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pubmed:meshHeading |
pubmed-meshheading:17140567-Animals,
pubmed-meshheading:17140567-Cell Death,
pubmed-meshheading:17140567-Cell Membrane Permeability,
pubmed-meshheading:17140567-Cells, Cultured,
pubmed-meshheading:17140567-Cysteamine,
pubmed-meshheading:17140567-DNA Damage,
pubmed-meshheading:17140567-Fibroblasts,
pubmed-meshheading:17140567-Gene Expression,
pubmed-meshheading:17140567-Genetic Vectors,
pubmed-meshheading:17140567-Humans,
pubmed-meshheading:17140567-Mice,
pubmed-meshheading:17140567-Peptides,
pubmed-meshheading:17140567-Recombinant Fusion Proteins,
pubmed-meshheading:17140567-Ribosomal Proteins,
pubmed-meshheading:17140567-Skin,
pubmed-meshheading:17140567-Ultraviolet Rays
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pubmed:year |
2006
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pubmed:articleTitle |
Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death.
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pubmed:affiliation |
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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