| pubmed-article:1713060 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C0596972 | lld:lifeskim |
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| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C0014274 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C0376451 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C0231881 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C1521743 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C0181904 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C1704646 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C1707310 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C0728873 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:1713060 | lifeskim:mentions | umls-concept:C0040664 | lld:lifeskim |
| pubmed-article:1713060 | pubmed:issue | 29 | lld:pubmed |
| pubmed-article:1713060 | pubmed:dateCreated | 1991-8-27 | lld:pubmed |
| pubmed-article:1713060 | pubmed:abstractText | Resonance energy-transfer approaches have been used to directly monitor the interactions of the GTP gamma S-bound alpha subunit of transducin (alpha T GTP gamma S) with the retinal cyclic GMP phosphodiesterase (PDE). The PDE was labeled with 5-(iodoacetamido) fluorescein (IAF-PDE) and served as the fluorescence donor in these experiments while the alpha T GTP gamma S was labeled with eosin-5-isothiocyanate (EITC-alpha T GTP gamma S) and served as the energy acceptor. The EITC-alpha T GTP gamma S species was able to quench a significant percentage of the IAF-PDE fluorescence (typically greater than or equal to 30%) due to resonance energy transfer between the IAF and EITC moieties. The quenching by the EITC-alpha T GTP gamma S species was dose-dependent, saturable (Kd = 21 nM), and specific for the GTP gamma S-bound form of the alpha T subunit. Limited trypsin treatment of the IAF-PDE, which selectively removes a fluorescein-labeled gamma subunit (gamma PDE), completely eliminates the quenching of the IAF fluorescence by the EITC-alpha T GTP gamma S complex. Although the EITC-alpha T GTP gamma S complex competes with the unlabeled alpha T GTP gamma S for a binding site on the IAF-PDE, as well as for a site on the native PDE, it is not able to stimulate PDE activity. Thus, the modification of a single EITC-reactive residue on the alpha T GTP gamma S complex prevents this subunit from eliciting a key activation event within the retinal effector enzyme. | lld:pubmed |
| pubmed-article:1713060 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1713060 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1713060 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1713060 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1713060 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1713060 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:1713060 | pubmed:author | pubmed-author:EricksonJ WJW | lld:pubmed |
| pubmed-article:1713060 | pubmed:author | pubmed-author:CerioneR ARA | lld:pubmed |
| pubmed-article:1713060 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1713060 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:1713060 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:1713060 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1713060 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1713060 | pubmed:pagination | 7112-8 | lld:pubmed |
| pubmed-article:1713060 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:meshHeading | pubmed-meshheading:1713060-... | lld:pubmed |
| pubmed-article:1713060 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1713060 | pubmed:articleTitle | Resonance energy transfer as a direct monitor of GTP-binding protein-effector interactions: activated alpha-transducin binding to the cGMP phosphodiesterase in the bovine phototransduction cascade. | lld:pubmed |
| pubmed-article:1713060 | pubmed:affiliation | Department of Pharmacology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853-6401. | lld:pubmed |
| pubmed-article:1713060 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1713060 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:1713060 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1713060 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1713060 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1713060 | lld:pubmed |
