Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1991-8-22
pubmed:databankReference
pubmed:abstractText
Lactococcus lactis subsp. cremoris is of considerable interest to the dairy industry, which relies upon the few available strains for the manufacture of cheddar cheese free of fermented and fruity flavors. The subspecies cremoris differs from related subspecies by the lack of a few phenotypic traits. Our purpose was to identify unique rRNA sequences that could be used to discriminate L. lactis subsp. cremoris from related subspecies. The 16S rRNAs from 13 Lactococcus strains were partially sequenced by using reverse transcriptase to identify domains unique to L. lactis subsp. cremoris. All five strains of the subspecies cremoris had a unique base sequence in a hypervariable region located 70 to 100 bases from the 5' terminus. In this region, all L. lactis subsp. lactis biovar diacetylactis strains examined had a sequence identical to that of L. lactis subsp. lactis 7962, which was different from other strains of the subspecies lactis by only one nucleotide at position 90 (Escherichia coli 16S rRNA structural model) (J. Brosius, J. L. Palmer, J. P. Kennedy, and H. F. Noller, Proc. Natl. Acad. Sci. USA 75:4801-4805, 1978). Oligonucleotide probes specific for the genus Lactococcus (212RLa) and for the subspecies cremoris (68RCa) were synthesized and evaluated by hybridization to known rRNAs as well as fixed whole cells. Efficient and specific hybridization to the genus-specific probe was observed for the 13 Lactococcus strains tested. No hybridization was seen with the control species. All five strains of the subspecies cremoris hybridized to the subspecies-specific probe.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-158748, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-16350018, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-2310179, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-2413450, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-2430518, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-2448289, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-2466341, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-2467178, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-2479630, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-2782871, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-3136142, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-3139049, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-368799, http://linkedlifedata.com/resource/pubmed/commentcorrection/1713027-4571084
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1313-8
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Development and application of oligonucleotide probes for identification of Lactococcus lactis subsp. cremoris.
pubmed:affiliation
Department of Microbiology, Oregon State University, Corvallis 97331-3804.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't