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rdf:type |
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lifeskim:mentions |
umls-concept:C0185023,
umls-concept:C0205360,
umls-concept:C0237497,
umls-concept:C0851285,
umls-concept:C1156019,
umls-concept:C1166785,
umls-concept:C1314972,
umls-concept:C1424628,
umls-concept:C1947904,
umls-concept:C1999228,
umls-concept:C2825781
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pubmed:issue |
5
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pubmed:dateCreated |
2006-11-28
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pubmed:abstractText |
Mitotic cells face the challenging tasks of linking kinetochores to growing and shortening microtubules and actively regulating these dynamic attachments to produce accurate chromosome segregation. We report here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability. Microinjection of an antibody to the N terminus of Hec1 suppresses both microtubule detachment and microtubule plus-end polymerization and depolymerization at kinetochores of PtK1 cells. Centromeres become hyperstretched, kinetochore fibers shorten from spindle poles, kinetochore microtubule attachment errors increase, and chromosomes severely mis-segregate. The N terminus of Hec1 is phosphorylated by Aurora B kinase in vitro, and cells expressing N-terminal nonphosphorylatable mutants of Hec1 exhibit an increase in merotelic attachments, hyperstretching of centromeres, and errors in chromosome segregation. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Biopolymers,
http://linkedlifedata.com/resource/pubmed/chemical/CLASP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/KIF2C protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kinesin,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NDC80 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/SPC24 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/aurora kinase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
127
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
969-82
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pubmed:dateRevised |
2011-7-11
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pubmed:meshHeading |
pubmed-meshheading:17129782-Amino Acids,
pubmed-meshheading:17129782-Antibodies, Monoclonal,
pubmed-meshheading:17129782-Biopolymers,
pubmed-meshheading:17129782-Epitope Mapping,
pubmed-meshheading:17129782-HeLa Cells,
pubmed-meshheading:17129782-Humans,
pubmed-meshheading:17129782-Kinesin,
pubmed-meshheading:17129782-Kinetochores,
pubmed-meshheading:17129782-Metaphase,
pubmed-meshheading:17129782-Microtubule-Associated Proteins,
pubmed-meshheading:17129782-Microtubules,
pubmed-meshheading:17129782-Mitotic Spindle Apparatus,
pubmed-meshheading:17129782-Models, Biological,
pubmed-meshheading:17129782-Nuclear Proteins,
pubmed-meshheading:17129782-Phosphorylation,
pubmed-meshheading:17129782-Protein Transport,
pubmed-meshheading:17129782-Protein-Serine-Threonine Kinases
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pubmed:year |
2006
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pubmed:articleTitle |
Kinetochore microtubule dynamics and attachment stability are regulated by Hec1.
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pubmed:affiliation |
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. jgdeluca@email.unc.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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